Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes. Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus (HCV) by cloning the gene of NS5ABP37 protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (α type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we made a sequence analysis by bioinformatics. Results We screened twenty-five proteins binding to NS5ABP37, including Homo sapiens cyclin Ⅰ (CCNI) gene, Homo sapiens matrix metallopeptidase 25 (MMP25) and Homo sapiens talin 1. Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV. And the biological function of NS5ABP37 may be associated with glycometabolism, lipid metabolism and apoptosis.     

解读《扫路车》标准

开栏的话: 标准至关重要。对各行各业,特别是专用车行业而言,标准的出台意味着各相关企业必须遵守标准对其所做的限制,否则其生产出来的相关产品将被视为不合格产品。因此,企业对标准的制修订非常关注,不仅因为标准的约束力,还因为标准在一定程度上引导着产品该朝哪个方向发展。本刊从这期开始开设《解读》栏目,将不定期地刊登解读我国专用汽车相关标准法规的文章,以期更好地为企业服务。如果您对我国专用汽车的标准制修订工作有哪些意见和建议,欢迎投稿。     

不具法律约束力的《哥本哈根协议》

2009年12月19日，联合国气候变化框架公约第十五次缔约方会议和京都议定书第五次缔约方会议在丹麦首都哥本哈根落幕，会议达成了不具法律约束力的《哥本哈根协议》。《哥本哈根协议》维护了《联合国气候变化框架公约》及其《京都议定书》确立的“共同但有区别的责任”原则，就发达国家实行强制减排和发展中国家采取自主减缓行动作出了安排，并就全球长期目标、资金和技术支持、透明度等焦点问题达成广泛共识。     

落实军车违章抄告制度应"三要"

要深入宣传教育，增强“三个意识”一是要深入学习，增强法规意识。知之深才能行之切，要确保抄告制度深入人心，使其对广大驾驶员具有较强的约束力，首当其冲的是要组织车勤人员反复学习、认真领会，切实弄懂弄通。要将抄告制度的主要内容以卡片形式打印出来，下发到全体驾驶员手中，随车携带、随时学习，使其树立制度就是法规的意识。     

中国海员发证体系通过审核

国际海事组织海洋环境保护委员会第53次会议前不久召开．会议同意采取“高度优先”的原则，制定一个有关拆船再循环的规则，以此为国际航运和再循环设施提供具有法律约束力和全球适用的船舶再循环法规。     

论班轮船期表的法律性质

班轮船期表在班轮运输实践中广泛应用,已形成了固定的班轮航运惯例。其载明的内容具体明确,也默示表示承受轮船期表的约束,依据合同法的规定,班轮船期表符合要约的法定要件,应是运输要约。班轮船期表中海上货物运输合同订立的重要程序和形式,对承托双立具有法律约束力。     

Analysis of chiral non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac binding with HSA

The protein binding of non-steroidal anti-inflammatory drugs flurbiprofen, ketoprofen and etodolac with human serum albumin （HSA） was investigated using indirect chiral high performance liquid chromatography （HPLC） and ultrafiltration techniques. S-（-）-1-（1-naphthyl）- ethylamine （S-NEA） was utilized as chiral derivatization reagent and pre-column derivatization RP-HPLC method was established for the separation and assay of the three pairs of enantiomer. The method had good linear relationship over the investigated concentration range without interference. The average extraction efficiency was higher than 85% in different systems, and the intra-day and inter-day precisions were less than 15%. In serum albumin, the protein binding of etodolac enantiomers showed significant stereoselectivity that the affinity of S-enantiomer was stronger than R-enantiomer, and the stereoselectivity ratio reached 6.06; Flurbiprofen had only weak stereoselectivity in HSA, and ketoprofen had no stereoselectivity at all. Scatchard curves showed that all the three chiral drugs had two types of binding sites in HSA.     

高职“工程力学”课程受力图教学研究

正确绘制物体受力图是学好工程力学的基础,也是解决工程中力学问题之关键。学生在学习此章节时,往往采用物理学中画受力图的方法来进行受力分析,从而导致作图错误。文章主要从改变传统四步作图法着手,通过对杆件受力的分析与研究,让学生掌握绘图的分析技巧,以达到快速掌控绘制受力图之目的。     

船员劳务合同纠纷案评析

书面承诺具有约束力,如无足以推翻该项书面承诺的相反证据,应依照约定履行义务。双方未约定保证期间的,保证期间应为主债务履行期届满之日起六个月。     

IMO通过减少碳排放量的条款

IMO关于减少二氧化碳排放量的规则条款几经周折终于获得通过，该条约的约束力不容忽视。IMO成员国达成共识将是一项艰巨的任务，看似“拼凑而成”的条款所发挥的效力远胜于没有条款或在联合国气候变化框架公约下进行的起草工作（因为IMO出台的相关措施更加能满足航运业的需求）。