胆石中游离和结合胆汁酸定量分析研究(薄层扫描色谱法)

在付立叶变换红外光谱(FT-IR)定性分类胆石的基础上,用薄层扫描色谱法进行胆石中游离和结合胆汁酸的定量分析研究。60例胆石经FT-IR 分为7种类型:胆固醇结石,胆固醇混合石、胆色素混合石、色素新型石、类色素型石、黑石磷酸盐型和黑石蛋白型。本文将从不同类型胆石游离和结合胆汁酸含量的差异,探讨胆汁酸在胆石成因中的作用.     

用PCR技术标记双链DNA探针

利用ＰＣＲ技术制备生物素或地高辛标记的ＤＮＡ探针，经原位杂交试验及敏感性和特异性检测，均取得满意效果。ＰＣＲ标记法具有经济、快速、简易和标记量大等优点。实践中发现Ｂｉｏ／Ｄｉｇ－１１－ｄＵＴＰ的质量、ｄＴＴＰ和Ｂｉｏ／Ｄｉｇ－１１－ｄＵＴＰ的比例及起始ＤＮＡ模板浓度是标记成败的关键。     

DIG－DNA探针检测宫颈癌组织中HPV－16的E_7转化基因

应用地高辛配基（Ｄｉｇ－１１－ｄＵＴＰ）标记人乳头瘤病毒１６型（ＨＰＶ－１６）Ｅ７转化基因作探针，分别检测了宫颈癌活检组织、宫颈炎及正常宫颈脱落细胞ＤＮＡ。其检出率为：宫颈癌中４２．１％（２４／５７）、宫颈炎中２．５％（１／３９）、正常宫颈中５．５％（１／１８）。宫颈组织中ＨＰＶ－１６Ｅ７转化基因的检出率较高，提示该转化基因与宫颈癌的发生可能密切相关。本研究也证实ＤＩＧ配基标记的Ｅ７基因探针具有特异、敏感、安全、稳定和能反复使用的优点，适合基层使用。     

慢性氟中毒对大白鼠免疫功能的影响

长期服用氟化钠水溶液后，对大白鼠进行免疫功能测试，结果证明用８８．５ｍｇ／Ｌ氟化钠水溶液服用３１５ｄ时，大白鼠的体液免疫反应（以血清凝集素和ＰＦＣ为指标）及细胞免疫反应（以Ｅ—玫瑰花结形成和ＡＮＡＥ为指标）均有抑制作用，尤其是对食用低营养食物的大白鼠，抑制作用更为显著。     

Investigating the Interaction Between Gluconobacter Oxydans and Bacillus M egaterium for 2-keto-L-gulonic Acid Biosynthesis in the Two-Step Vitamin C Fermentation

In the two-step vitamin C fermentation process, its precursor 2-keto-L-gulonic acid was synthesized from L-sorbose by mixed culture of Gluconobacter oxydans and Bacillus megaterium. The interaction between Gluconobacter oxydans and Bacillus megaterium remains unclear and it is a challenge to mathematically model the mixed growth of these two strains. The Monod-type equations were previously proposed to describe the coupled growth of Gluconobacter oxydans and Bacillus megaterium. However, in this study, we modeled the interaction of these two strains in a macroscopic view by introducing the population theory. Taking account of the fact that the density or concentration of Gluconobacter oxydans or Bacillus megaterium was hardly to measure accurately in the mixed culture broth, the data of concentrations of the substrate and product were used to indirectly investigate the relation between these two strains. Three batch experiments were used to validate our model. And according to the values of identified parameters, the type of interaction between Gluconobacter oxydans and Bacillus megaterium was concluded to be predation, where Gluconobacter oxydans was predator, and Bacillus megaterium was prey.     

新装修居室空气中甲醛污染状况的研究

选取 18户新装修居室 ,通过室内连续定期定点采样 ,用变色酸法测定 ,研究了新装修材料影响室内甲醛含量增加的因素 ,根据室内甲醛浓度 -时间动力学曲线 ,提出了合理的搬迁时间。     

Comparative pharmacokinetics of chlorogenic acid after oral administration in rats

The present study was aimed at the comparison of the pharmacokinetics of pure chlorogenic acid and extract of Solanum lyratum Thunb. The animals were allocated to two groups, and were administered chlo...     

玄武岩纤维材料在酸、碱性环境下的耐久性试验研究*

为了解玄武岩纤维本身的耐酸、耐碱性能,对其在80℃2 mol/L盐酸和氢氧化钠溶液中腐蚀不同周期后的质量变化、单丝强度进行了试验研究,并与聚丙烯纤维、耐碱玻璃纤维的耐酸碱性进行了对比。结果表明,在耐酸性能方面,玄武岩纤维最优,其次是聚丙烯纤维,耐碱玻璃纤维表现最差;在耐碱性能方面,表现优劣依次为聚丙烯纤维、玄武岩纤维和耐碱玻璃纤维。试验结果为玄武岩纤维新材料在水泥混凝土中的推广应用提供参考。     

Improvement of oil/water selectivity by stearic acid modified expanded perlite for oil spill cleanup

Ecological disasters and economic losses resulting from oil spills have reminded us the necessity for finding an environmentally friendly, cost-effective, and available in large-scale materials to minimize the oil spill effects. The development of high oil/water selectivity mineral sorbent for the removal of oil from the water is of great interest for oil spill cleanup. In this work, highly oil/water selectivity sorbent of expanded perlite modified with stearic acid was prepared by solution-immersion processes, and the characterization of sorbent was analyzed by Fourier transformation infrared (FT-IR) spectroscopy technique. The optimized amount of loaded stearic acid (SA) on the expanded perlite surface was found to be 2.0%. Then the sorption characteristics of unmodified expanded perlite (UMEP) and stearic acid modified expanded perlite (SMEP) were tested. The effects of different particles size, oil/water volume ratio and sorption simulated condition were investigated. It was found that expanded perlite modified with stearic acid could adsorb selectively oil from water in oil/water system. The maximum oil/water sorption ratio of SMEP was 46, which was about 191 times that of UMEP (0.24) in studied experimental range. The FT-IR analysis demonstrated the presence of long-chain alkyl group in the SMEP samples, which played an important role in oil/water selectivity of SMEP samples. The equilibrium were tested with Langmuir and Freundlich isotherm models, and oil sorption process of SMEP showed good correlation with the Langmuir isotherm model.     

BIOCHEMICAL STUDIES ON GLUCOAMINOGLYCANS CONTENT IN AFFECTED CARTILAGE HYALURONIC ACID LEVELS IN SERUM AND SYNOVIAL FLUID OF IDIOPATHIC FEMORAL HEAD NECROSIS

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