燃料电池电动车车载甲醇重整器

对目前世界几大汽车公司研制开发的燃料电池电动汽车车载甲醇重整器加以介绍，着重讨论了重整器的设计与重整催化剂的布置。指出，甲醇重整器的效果良好，但重整器出口富氢重整气中CO的含量仍然很高，而较高浓度的CO会使燃料电池电极中毒。因此，从重整器中产生出来的重整气在进入燃料电池以前要进行净化。     

The symbolism of ‘eco cars’ across national cultures: Potential implications for policy formulation and transfer

Transport choices are not merely practical decisions but steeped in cultural and societal perceptions. Understanding these latent drivers of behaviour will allow countries to develop and import policies to more successfully promote sustainable transport. Transport symbolism – what people believe their ownership or use of a mode connotes to others about their societal position – has been shown to be one such, non-trivial, hidden motivator. In the case of hybrid and electric cars (‘eco cars’), studies have demonstrated how their symbolic value varies within a society among different social groups. As yet, however, there has been scant research into comparing how the symbolism of a mode varies across national cultures, horizontally, between individuals with similar socio-demographic characteristics. Through qualitative thematic analysis, this study utilises two of Hofstede’s cross-cultural indices – power differential and individualism versus collectivism – to develop and strengthen theory on how the differing symbolism of eco cars currently varies between four cultural clusters – Anglo, Nordic, Confucian and South Asian. It also deliberates how observed symbolic qualitative differences may influence an individual or group choice to procure eco cars. Finally, it discusses how policy development, transfer and marketing, within the context of eco cars, may need to be modified by national governments, in the Confucian and South Asian cultures, so as to encourage uptake and modal shift.     

新型混凝土单元块D型软体排结构计算及稳定性分析

针对长江中下游航道整治水下沉排工程中软体排结构的不足,提出一种新型混凝土单元块D型软体排结构,建立悬链线模型,分析排体的受力特点,计算排体的抗倾、抗滑和抗漂浮稳定性,并对比分析排体对地形的适应性。结果表明,新型混凝土单元块D型软体排结构明显提高了水下沉排的安全性和对复杂地形的适应性,为复杂条件下的水下沉排工程提供理论支撑。     

Career development for women in maritime industry: organisation and socialisation perspectives

For centuries, the maritime industry has been a recognised place in society dominated solely by men, and even though there were women involved, they were mostly on the periphery. This research aims to fill the gap of women’s career development in this field, by investigating the relationships among the degree of organisational socialisation, women’s workplace culture, and the career development of women in the maritime workplace. The analyses are conducted with the structural equation model with 202 valid questionnaires. The results indicated that women’s workplace culture has a significantly negative relationship with the degree of organisational socialisation, and then in turn, it has a significantly positive relationship with women’s career development in the maritime industry. Finally, the results also indicate that the mediating effect of organisational socialisation existed between women’s workplace culture and women’s career development. According to the empirical analysis, this study makes conclusions as well as suggestions for the maritime enterprises as the reference for human resource management.     

体外大鼠成骨细胞对破骨细胞形成的影响

目的通过将鼠成骨细胞与破骨细胞直接共培养的实验方法,研究成骨细胞对破骨细胞分化及功能成熟的影响。方法按照M-CSF 30μg/L、RANKL 50μg/L的浓度对鼠骨髓单个核细胞诱导培养6 d,与原代培养3 d的鼠成骨细胞按细胞数量1∶1直接共培养,加入1,25-(OH)2D31×10-8mol/L和PGE21×10-6mol/L,利用形态学观察、TRAP染色、骨吸收陷窝检测等方法对共培养细胞进行鉴定。结果成骨细胞与破骨细胞共培养时,成骨细胞有明显的生长优势;染色后显微镜下可见大量呈单层排列的成骨细胞,偶见TRAP( )的破骨细胞。结论成骨细胞对破骨细胞分化及功能成熟的影响与两者的相对数量有关。本实验中,由于成骨细胞快速生长,当其数量多于破骨细胞时,可能使1,25-(OH)2D3对成骨细胞RANKL表达的上调作用不足,且PGE2对成骨细胞OPG表达的下调作用不足,从而主要表现为对破骨细胞形成及分化的抑制作用。     

羟基喜树碱对人胆管癌QBC939裸鼠移植瘤PS2、COX-2的影响

目的探讨羟基喜树碱对人胆管癌QBC939裸鼠移植瘤的乳癌相关肽(PS2)、环氧合酶-2(COX-2)的影响,分析PS2、COX-2与肿瘤发生、发展的关系。方法建立人胆管癌QBC939细胞株裸鼠皮下移植瘤模型,将30只裸鼠随机分成对照组、羟基喜树碱低剂量组和羟基喜树碱高剂量组,观察移植瘤体生长情况,RT-PCR法检测瘤体内PS2、COX-2 mRNA表达。结果与对照组比,羟基喜树碱低、高剂量组都可使移植瘤体的PS2 mRNA表达增高;COX-2 mRNA表达降低,且瘤体生长减慢,与对照组比较差异有统计学意义(P<0.01)。结论羟基喜树碱可降低移植瘤COX-2 mRNA表达,增高移植瘤体的PS2 mRNA表达,并使人胆管癌裸鼠移植瘤生长减慢。     

生存素在5-氟尿嘧啶诱导人绒癌细胞株JAR凋亡中的作用

目的探讨生存素(survivin)在化疗药物5-氟尿嘧啶(5-Fu)诱导的人绒癌细胞株(JAR)凋亡中的作用。方法用JAR细胞株进行培养传代,以不同浓度5-Fu作用于JAR细胞,MTT方法观察5-Fu对JAR细胞生长的抑制情况,流式细胞仪检测细胞凋亡率,用逆转录聚合酶链(RT-PCR)和免疫印迹技术(Western-blot)方法检测survivin基因和蛋白的表达。结果①5-Fu对JAR细胞的生长具有抑制作用,并有浓度和时间的依赖性;②5-Fu诱导JAR细胞凋亡,凋亡率随浓度而增加;③5-Fu作用后survivin基因和蛋白表达减少,随浓度增加而减少。结论5-Fu可能是通过降低survivin表达水平,诱导JAR细胞凋亡。     

刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。     

正常绒毛和葡萄胎中Mel-CAM标记中间型滋养细胞的增殖情况

目的探讨葡萄胎中Mel-CAM与滋养细胞黏附、浸润之间的关系以及Mel-CAM标记中间型滋养细胞(IT)的增殖情况。方法采用双重免疫组化法,检测Mel-CAM标记IT的Ki-67表达情况。结果葡萄胎中Mel-CAM表达较正常绒毛明显增高,差异有统计学意义(P<0.05);葡萄胎绒毛表面有Mel-CAM标记IT出现,而正常绒毛表面则无;葡萄胎组织中IT的Ki-67指数较正常绒毛明显增高,差异有统计学意义(P<0.05)。结论葡萄胎绒毛表面有Mel-CAM表达,表现出双重分化方向,这可能与葡萄胎的发病机制有关;检测葡萄胎组织中IT的Ki-67指数对于预测葡萄胎的转归有潜在的价值。