如何发展供应链企业间的信任关系

探讨了在供应链间相互竞争的商业模式下,供应链企业间发展相互信任关系的策略问题。     

人B7.2(CD86)胞外区原核表达及其工程菌发酵培养的实验研究

目的　利用原核系统表达人B7.2 (IgV +C)并对工程菌发酵培养条件进行优化。方法　用聚合酶链反应 (PCR)技术从B7.2cDNA全长中克隆B7.2 (IgV +C) ,将PCR产物克隆入表达载体 pGEX 4T 3从而得到重组体 pGEX 4T 3 /hB7.2 (IgV +C) ,SDS PAGE及Westernblot用于检测目的蛋白的表达 ,同时对目的蛋白诱导表达时间及诱导剂浓度进行优化 ,对重组质粒的遗传稳定性进行鉴定。结果　Westernblot结果显示相应分子质量 5 5kD处有hB7.2 (IgV+C)与GST融合蛋白的高效表达 ,表达量占菌体总蛋白的 3 0 %左右。对工程菌进行发酵培养研究的结果表明 ,所构建的重组质粒在工程菌DH5α中传代稳定 ,未见质粒丢失 ,目的蛋白的表达不受影响 ,且在 3 7℃诱导 5h、IPTG终浓度为 4mmol·L- 1 时其表达量最多。结论　证明了利用原核系统表达人B7.2 (IgV +C)的可行性。     

ALTERATION IN CONTRACTILE RESPONSE TO NORADRENALINE,5-HYDROXYTRYPTAMINE, SARAFOTOXIN 6c, AND ANGIOTENSINⅡ IN RAT MESENTERIC ARTERY DURING ORGAN CULTURE

Theresistanceinindividualvascularbedsdeter minesthelocaldistributionofbloodflowtoandwithindifferentorgans.Thecontractilestateofanarteryiscontrolledbymediatorsreleasedlocallyfromnerveterminalsandendothelialcells ,andbycirculatinghormones .Thesemediatorsgiverisetoeffectsthroughtheirreceptors .Mediatorscanaffectvascularsmoothmuscleei therdirectly ,byactingonthesmoothmusclecellsthemselves ,orindirectly ,byactingonendothelialcellsoronnerveterminals ,especiallythoseofthesympatheticnervoussystem .Som…     

ALTERATION IN CONTRACTILE RESPONSE TO NORADRENALINE,5-HYDROXYTRYPTAMINE,SARAFOTOXIN 6c,AND ANGIOTENSINⅡIN RAT MESENTERIC ARTERY DURING ORGAN CULTURE

Objective To compare the vasoconstrictive effects of 9 mediators on fresh and incubated mesenteric arteries of rats. Methods The superior mesenteric artery of rat was removed and the endothelium was denuded. The vessels were cut into 1 mm long cylindrical segments and subjected to organ culture for 24 hours. Fresh or incubated segments were immersed into tissue baths and the concentration-response curves were obtained by cumulative administration of the vasoconstrictors. Results In fresh mesenteric artery, endothelin-1 (ET-1), 5-hydroxytryptamine (5-HT), noradrenaline (NA), 5-carboxamidotryptamine (5-CT), and angiotensinⅡ (AngⅡ) induced potent and sustained constrictions in a concentration-dependent manner. The contraction induced by sarafotoxin 6c (S6c) was weak, while bradykinin (BK), des-Arg-bradykinin (DA-BK), and human urotensinⅡ (hUT-II) showed no detectable contraction. The concentraion-response curves in order of slopes was ET-1, NA, 5-HT, 5-CT, and AngⅡ. The order of the maximum contractions was ET-1>NA=5-HT=5-CT>AngⅡ>S6c. After organ culture, the concentration-response curves induced by S6c, NA, and 5-HT were significantly increased, while that induced by AngⅡ was decreased as comparing to fresh arteries. BK contracted the artery only weakly. Conclusion Organ culture changed the phenotypes towards an increased efficacy of NA, 5-HT, S6c, and a reduced efficacy of AngⅡ, which is in accordance with the results of pharmacological characterization in some human vascular disease.     

ALTERATION OF HB-EGF IN PSORIATIC EPIDERMIS WITH THE TREATMENT OF RETINOID ACID

Retinoidacid (RA)bindingtoitsnuclearre ceptorscouldexertawidevarietyofprofoundef fectsininhibitingproliferationofepidermis ,induc ingdifferentiation ,maintainingthemorphologicalfeatureofnormaltissues ,influencingblastocystde velopment,formationoforgansandtumors .Psori asisischaracterizedbyabnormalkeratinocyteprolif eration ,terminaldifferentiationanddermalangio genesis .Heparinbindingepidermalgrowthfactor likegrowthfactor (HB EGF) ,asamemberofepi dermal growthfactor (EGF )family ,hasbeenpr…     

轨道交通站点慢行接驳一体化研究

城市轨道交通效益的发挥离不开与其他交通方式的有效衔接。现阶段,以非机动车为主的慢行交通方式越来越受到人们的重视。结合常州地铁1号线新桥站的接驳现状,借鉴国内外相关城市慢行轨道换乘站点的规划理念,在慢行接驳换乘优先的理念下,对常州市新桥站进行了站点一体化接驳的规划研究。     

变坡横移纵向滑道在叙利亚塔尔图斯修理厂工程的应用

回顾和总结我国外经委援助工程叙利亚塔尔图斯修理厂工程的水工设计,简要介绍变坡横移计算的原理.工程由纵向船排滑道、变坡横移区和水平船台组成.变坡横移滑道工程上排船质量达1 200 t,船排首小车承载质量达300 t,变坡横移架载质量达1 200 t,在宽阔水域中,定位栈桥的应用提高了船舶上排的操作效率,确保滑道下水上排操作的安全.变坡横移纵向滑道设计技术达到国际先进水平.     

内皮和平滑肌相互作用与动脉粥样硬化发生机制的研究进展

西方国家的死亡原因中，由动脉粥样硬化（Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ，Ａｓ）引起的心脑血管疾病占首位［１］。近年来，随着我国人民生活水平逐步提高，该病在我国的发病率和死亡率也逐年上升。动脉粥样硬化发病隐袭，进展缓慢，病期长，发病机制十分复杂。攻克动脉粥样硬化防治难关的关键是弄清血管细胞生物学的基本病理生理改变。动脉内皮细胞（ｅｎｄｏｔｈｅｌｉａｌｃｅｌｌｓ，ＥＣ）和平滑肌细胞（ｓｍｏｏｔｈｉｎｎｓ－ｅｃｅｌｌｓ，ＳＭＣ）在动脉粥样硬化发生和发展中扮演着重要角色［２］。在“危险因素”氧化型低密度脂…