新大洋里的“女伯爵”

坠毁后的发现 “连接!”皮特坐在驾驶座上用大家都懂的英语和俄语各说了一遍。约那斯不需解释就明白他说的是什么意思:与拜科努尔的联系中断了。在接下去的二十分钟内他们就应该降落了,燃料已快用完,气流的干扰迫使他们不断地修正轨道,被迫比计划提前二圈进行着陆。他们先前还希望拜科努尔能给他们提供一个完好的飞机场,可眼下在他们底下出现的只有阿尔卑斯     

上海城铁明珠线的集中维护系统

轨道交通通信系统包含的子系统比较多，一般情况下有传输、广播、电视监控、电源、公安无线、消防无线、时钟系统、专用电话等。面对如此多的系统，如果进行分散管理，既浪费人力，又会使一些故障不能及时发现而影响系统修复。为此，采用集中维护系统，使所有故障集中反映到集中网管平台上，便可安全、有效地使用通信系统。     

铁路物资企业信息化改造浅析

以某铁路物资企业为背景,基于铁路物资企业的业务特点及未来的发展需要提出该类企业信息化改造的方案建议.     

非正常行车机车操纵模拟系统的研究

总结机务非正常行车的主要情况,分析既有非正常行车机车操纵教学模拟系统存在的问题,提出基于SS4G电力机车模拟操纵台非正常行车操纵教学系统的设计思路和技术方案,归纳该系统的技术特点,指出该系统的改进方向。     

高速公路机电工程安装与质量检测探析

结合某高速公路机电工程实例,以工程概况作为切入点,分析了该高速公路机电工程的安装技术,并探讨了高速公路机电工程安装与质量检测措施,以优化整个机电工程系统。     

Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

增压发动机泄气声品质的研究及控制方式探讨

为解决采用涡轮增压器后带来的收油泄气声品质问题,对问题工况进行测试,并确定收油异响的辐射源。通过对泄压阀工作原理进行研究,确认该异响为泄压阀泄气不及时造成的压气机瞬时喘振,从而形成噪声。最后,采用将泄压阀弹簧刚度调低,减小泄压阀开启压力的方式,使泄压阀及时开启泄压,消除该噪声。     

变形车辆自标定三维重建的建模研究

变形车辆测量是道路交通事故再现的重要基础性工作。建立基于普通数码相机的事故变形车辆自标定三维重建模型,可以避免在现场设置标定物,有利于提高变形车辆的勘测效率和三维重建设备的使用方便性。研究了基于基础矩阵估计的事故变形车辆摄影图像自标定三维模型,并分析了其实现过程。实验表明,自标定三维重建能为事故再现提供详细的车辆变形描述。