大跨度三主桁连续钢桁梁桥受力特性研究

为研究横向构件布置与截面设计对3主桁受力均衡性的影响,以宁波市三官堂大桥主桥160m+465m+160 m=785 m的大跨径钢桁架连续梁桥为例,采用Midas/Civil软件建立钢桁架梁模型,分析比较对称荷载与偏载作用下主桁结构支反力、轴力和位移等静力效应,得出了3主桁连续钢桁梁桥的内力分布特性.     

Construction and characterization of hGM-CSF-expressing K-562 cell line

Objective The whole process of vaccine preparation is time-consuming and technically challenging. Here the hGM-CSF-engineered K-562 cell line was constructed to simplify tumor vaccine preparation process. Methods The eukaryocyte expressing plasmid pcDNA3. 1/GM-CSF was first constructed and its accuracy was verified through sequencing. The pcDNA3. 1/GM-CSF was transfected into COS-7 cells to verify GM-CSF expression and cytokine activity using TF-1 cell line. Then the plasmid was transfected into K-562 cell line using liposome method, and was selected under G-418 and sub-cloned by limiting dilution. GM-CSF product from the monoclone GM-CSF-K-562 cell lines was quantified using ELISA method. Results We successfully constructed the hGM-CSF eukaryocyte expressing plasmid and hGM-CSF expressing K-562 cell line. Conclusion The construction of K-562/GM-CSF line will simplify the preparation of tumor vaccine, thus facilitating the application of tumor vaccination therapy in clinical application.