生存素在5-氟尿嘧啶诱导人绒癌细胞株JAR凋亡中的作用

目的探讨生存素(survivin)在化疗药物5-氟尿嘧啶(5-Fu)诱导的人绒癌细胞株(JAR)凋亡中的作用。方法用JAR细胞株进行培养传代,以不同浓度5-Fu作用于JAR细胞,MTT方法观察5-Fu对JAR细胞生长的抑制情况,流式细胞仪检测细胞凋亡率,用逆转录聚合酶链(RT-PCR)和免疫印迹技术(Western-blot)方法检测survivin基因和蛋白的表达。结果①5-Fu对JAR细胞的生长具有抑制作用,并有浓度和时间的依赖性;②5-Fu诱导JAR细胞凋亡,凋亡率随浓度而增加;③5-Fu作用后survivin基因和蛋白表达减少,随浓度增加而减少。结论5-Fu可能是通过降低survivin表达水平,诱导JAR细胞凋亡。     

EZH2基因在宫颈癌中的表达及其意义

目的研究EZH2(enhancer of zeste homolog 2,EZH2)基因在宫颈癌组织中的表达及其意义。方法采用RT-PCR方法检测20例正常宫颈组织和60例宫颈癌组织中EZH2mRNA的表达情况,并结合临床资料分析其与宫颈癌各临床病理特征的相关性。结果 EZH2mRNA在宫颈癌组织中的表达高于正常宫颈组织(0.86±0.17 vs.0.06±0.02,P<0.01),EZH2mRNA表达与患者年龄及肿瘤大小无明显相关性(P>0.05),而与肿瘤细胞分化、临床分期、浸润深度及淋巴结转移密切相关(P<0.05)。结论 EZH2基因表达增强与宫颈癌恶性生长及不良预后有关。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

层黏连蛋白及其受体在正常绒毛和绒癌组织中的表达及意义

目的探讨层黏连蛋白(LN)及层黏连蛋白受体(LN-R)与滋养细胞侵袭的关系。方法采用免疫组织化学的方法,观察正常早期妊娠绒毛和绒癌组织中LN、LN-R的表达。结果在正常绒毛组织中从绒毛、细胞柱到浸润的绒毛外滋养细胞,随着滋养细胞侵袭力的增加,LN及LN-R的表达呈下降趋势。绒癌组织的LN表达较正常绒毛明显减弱,两组间比较有显著性差异(P<0.05)。结论在滋养细胞侵袭的过程中,LN和LN-R起着重要作用。