Monitoring trace elements (Al, As, Cr, Cu, Fe, Mn, Ni and Zn) in deep and surface waters of the estuary of the Nerbioi-Ibaizabal River (Bay of Biscay, Basque Country)

Deep water samples (in contact with the sediment) were collected at eight different points of the estuary of the Nerbioi-Ibaizabal River (Bay of Biscay, Basque Country), both at low and high tides, during four sampling campaigns (May, September and December 2005 and March 2006). Superficial water was also sampled in March 2006. Temperature, pH, redox potential, dissolved oxygen and electrical conductivity corresponding to each sample were measured in situ at each sampling point using a multiparametric probe. The physico-chemical parameters found are typical of highly stratified estuaries, with an acceptable oxygenation level. After filtering and acidifying the samples, they were analysed by inductively coupled plasma/mass spectrometry (ICP/MS) to simultaneously determine the total concentration of Al, As, Cr, Cu, Fe, Mn, Ni and Zn. Concentrations in the μg kg^− 1 level were found in all cases (c_Cr and c_Ni, 1–10; c_Al, c_As and c_Zn, 10–50; c_Cu and c_Mn, 10–100 and c_Fe, 100–400 μg kg^− 1). A probable net input of Al, Cr, Mn and Zn via the main (Nerbioi-Ibaizabal) and some of the tributary rivers (Galindo, Asua and Gobela) was identified. Evidence of a common source of Al and Zn to the estuary was found. Correlation analysis of data revealed connections between variables (concentration of Cu, Fe and As with salinity, as well as c_Al with c_Zn, c_Cu with c_Fe, c_As with c_Fe, and c_As with c_Cu). Principal Component Analysis (PCA) of data allowed the samples to be grouped according to sampling campaign, with two principal components accounting for 62% of the total variance. In addition, plots of element concentration against salinity suggested a conservative behaviour for As, Cu and Fe and a non-conservative one for Cr. Not clear mixing behaviour was observed for the rest of elements.     

Social marginal cost pricing and second best alternatives in partnerships for transport infrastructures

According to the economic theory, if all the first-best conditions are met, social optimality involves the prices to be set equal to social marginal costs. When it is not possible to set prices equal to social marginal costs, due to the presence of constraints within the transport sector or distortions elsewhere in the economy, the theory suggests corrections to the SMC principle (second-best alternatives). But the implementation of second best alternatives can give rise to serious problems when transferred from theory to practice. This chapter will discuss in particular the problems that might rise when investment cost are included (totally or partially) in the social marginal costs rules, and in particular when private operators are involved.     

A field study of the effectiveness of sacrificial anodes in ballast tanks of merchant ships

Sacrificial anodes have become a standard practice for the protection of ballast tanks of merchant vessels against corrosive damage. A well protected tank should extend the life span of a ship and consequently enhances its economic value. An in situ survey comprising more than 100 merchant vessels provided the opportunity to measure the impact of these anodes on the life expectancy of these vessels. Contrary to the general belief of these anodes’ beneficial effect, no significant difference was found in our observations in terms of corrosion occurrence between ship populations with and without sacrificial anodes, across all ship ages. This may be explained by the highly variable conditions and the complex geometry in a ballast tank severely impede optimal and straightforward installation of these anodes in these tanks. Also, poorly placed anodes in it may harm the integrity of the coating of the tank. We therefore plead for uniform and clear rules on anode installation and inspection.     

Assessing cruise port of call performance: a passenger-based approach using PLS modelling

In the present context of booming cruise demand, the study aims to advance the knowledge on port of call performance from cruise passengers’ perspective. The aim of this investigation was twofold: (1) to examine the structural links among port of call perceived quality–satisfaction–future intentions (revisit and word-of-mouth (WOM) behaviour); and (2) to test the moderating effect of cruisers’ motivation (push versus pull factors) to visit the port of call on the proposed structural links. Partial least squares path modelling was used to test the proposed model with a sample of 492 cruise passengers at a Mediterranean port of call. The findings revealed that port of call quality has a positive impact on port of call satisfaction, which in turn, strongly affects revisit and WOM intention. Besides, the results of the multi-group analysis indicated that the links between port of call quality–satisfaction and port of call satisfaction–WOM are moderated by cruisers’ motivation. Implications for port authorities and cruise lines are discussed.     

Mussel and Seaweed Cultivation in Offshore Wind Farms: An Opinion Survey

German coastal regions and adjacent offshore waters are claimed for a variety of different, and often overlapping, uses. Conflicts of interest are expected to increase due to the planned construction of offshore wind farms. The development of an integrated approach combining different commercial activities, such as open ocean aquaculture and wind farming, could be a possible solution to overcome these conflicts. To illuminate existing perception and opinions on this multiple-use idea, this study examines the attitudes of representatives of eight different actor groups toward potential offshore wind farm–mariculture integration in the German North Sea. Results from our questionnaire survey show that the respondents’ attitudes toward the suggested multiple-use setting seem to be largely influenced by their general opinion toward offshore wind farms. The Fisheries actor group differs from the other questioned groups in that negative attitudes are overall predominant. This case study points to the need for a credible mechanism that more successfully integrates key actor groups, such as fisheries, into future planning and research issues.     

Applying social marginal costs pricing to the road sector

This chapter, following the results of the case studies analyzed in the Enact project, will identify and analyze the implications of the possible application of SMCP in PPP’s in the road sector. The main issues analyzed include SMCP revenue formation and its ability to finance the PPP. The paper will focus on market and competition issues like: 1) the problems due to mispriced substitutes; 2) the interdependencies between the tolls and the capacity of different road infrastructures when these are competing for the same demand; 3) since short run social marginal costs do not repay for the investments costs (except in special cases), in the case tolls should cover also the investment costs this will lead to totally different pricing schemes between roads in a same area, with problem of demand shift toward cheaper existing infrastructures, therefore increasing the problem of cost recovery. The incentives caused by the introduction of prices based on SMC’s are also investigated.     

EFFECT OF LOW SELENIUM ON CHONDROCYTE DIFFERENTIATION AND DIFFERENTIAL EXPRESSION OF COLLAGEN TYPES Ⅰ , Ⅱ AND X IN ARTICULAR CARTILAGE FROM MINI-PIGS

Objective According to the distribution of low selenium areas, low nutrition state of the residents and the affecting cartilage growth and articular cartilage of Kashin-Beck Disease(KBD),the chondrocyte differentia- tion and differential expression of collagen types Ⅰ , Ⅱ and Ⅹ in articular cartilage from Chinese mini-pigs treated with low selenium were investigated in order to gain insight into the effects of these conditions on chondrocyte differ- entiation in KBD cartilage. Mothods Eleven male juvenile mini-pigs, aged from 4 weeks to 6 weeks after birth, were divided into 3 groups. The Se content in the diet of the “low Se” group was 0. 035mg/kg diet, and 0. 175 mg/kg diet in the control. For Se-supplemented group 0. 390mg /kg diet was added. The content of Se in blood was assayed at the beginning and at the end of each experiment. Samples of articular cartilage were taken from the right femur condylus, and collagen types Ⅰ , Ⅱ and Ⅹ in articular cartilage were analyzed by immunohistochemistry and in situ hybridization. Results ①All cartilage samples from juvenile mini-pigs fed with low selenium diet revealed a re- duction in type Ⅹ collagen mRNA expression in the hypertrophic chondrocytes as shown by in situ hybridization, and reduced type Ⅹ collagen deposition in the lower hypertrophic zone as shown by immunohistochemistry. ②Addition of selenium to the diet restored the type Ⅹ collagen to normal level. ③Type Ⅱ collagen was evenly distributed over the entire articular cartilage in all experimental and control groups. Type Ⅱ collagen mRNA signals were most prominent in the upper articular layer as well as in the hypertrophic zone in all groups. Type Ⅱ collagen expression was restrict- ed to the zone of endochondral ossification in all experimental groups and the control. Conclusion Low selenium has an down-regulatory role on the synthesis and deposition of collagen type Ⅹ in hypertrophic chondrocytes in articular cartilage of mini-pigs. Supplement of the low Se diet with additional Se restored the signals of collagen type Ⅹ to nor- mal levels. These findings indicate that selenium deficiency may disturb chondrocyte differentiation to hypertrophic cells in the growth plate,and worthy to be investigated further.     

The expansion of the Panama Canal and its impact on global CO2 emissions from ships

As part of the discussions of the environmental effects of the expansion of the Canal through the construction of a third set of locks, the Panama Canal Authority (PCA) began to consider measuring the impact of the project on global CO₂ emissions in 2006. The hypothesis PCA intends to investigate whether the third set of locks will prompt reductions in total world CO₂ emissions or not. The Canal's third set of locks will prevent reaching the saturation point, and will avoid diversion of traffic to potentially longer alternative routes, such as the Suez Canal and Cape Horn, thereby reducing distances and fuel consumption. In addition, the widening of the Canal may promote the construction of modern-type post-Panamax vessels, making transportation of freight more efficient through economies of scale. This white paper will initiate an exploratory research on the subject based on two possible scenarios: an existing Canal and an expanded Canal. Both scenarios will take physical distances, closest alternative routes, as well as fuel consumption of vessels and other relevant modes of transportation into consideration.