地下工程注浆技术研究新进展

注浆是土木、水利和矿山等工程建设中重要的防渗和加固技术。文章基于国内外注浆技术研究文献,总结了水泥等颗粒性浆液的“渗滤效应”及“黏度时空变异性”等理论研究成果,论述了非水反应高聚物、CW环氧树脂和微生物菌液等注浆新材料的应用现状及发展前景,介绍了细观力学数值模拟研究的突破性进展,可为地下工程注浆技术发展提供借鉴和启发作用。     

健美操选项课学生创新能力培养的研究与实践

采用文献资料法、问卷调查法、测量法、比较研究法、数理统计等方法,对辽宁省交通高等专科学校2014级健美操选项课学生进行调查,旨在掌握其创新能力现状、存在的问题、产生的原因,为采取干预对策,优化创新能力培养途径、方法,提高其创新能力提供科学依据。     

Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption

A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-l     

The clinical analysis of three methods in the treatment of intracranial bacterial infection

Objective To analyze the effect of three therapeutic methods to find an optimal approach to the treatment of intracranial bacterial infection by retrospectively reviewing 33 intracranial bacterial infection patients who were admitted from 1995 to 2008 in oar hospital. Methods The treatments by intermittent lumbar puncture,continuous lumbar subarachnoid space drainage, and embedment of Ommaya cyst for continuous drainage from the ventricles were performed in 15 cases, 12 cases, and 6 cases respectively along with intravenous application of full dose of antibiotics. Results Nineteen cases were cured and the best prognosis was from the group of Ommaya cyst embedment and continuous drainage from the ventricles. Conclusion Management goals are prompt recognition of the central nervous system (CNS) infection, rapid identification of causative organisms and initiation of treatment with the optimal management methods for complications. Embedment of Ommaya cyst for continuous drainage from the ventricle is a safe and effective treatment for intracranial bacterial infection.     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

加强综合素质培养成就个人事业

大学生学习生活是青年学生人生的关键阶段,不仅要学好规定的教学任务,更要加强综合素质培养,为终身学习打下良好基础,从容走向社会.     

农村创业人才培养的探索与实践

农业职业技能开发与创业人才培养是农村经济增长的关键。教育部为了有效解决农村人才短缺问题出台了"一村一名大学生计划",旨在培养新农村建设的带头人,从而更好地解决"三农"问题。     

结合行业特点，注重学生隐性能力培养的英语教学探索

文中基于隐性能力的内涵及其意义,分析了交通行业的岗位职责对学生隐性能力方面的要求,阐述了在英语教学中的应用实践及成效。笔者认为,行业的大发展离不开高素质人才的培养,包括隐性能力的培养,应该重视学生隐性能力的培养。     

汽车服务工程专业后市场技术领域“4341”人才定位

在企业和高校调研基础上,为实现人才培养与行业及社会发展需求相适应的目标,将我校汽车服务工程专业人才培养定位与汽车生产企业售后服务岗位对接,文章根据汽车服务人才金字塔式结构特点,初步确立了汽车后市场技术领域的高级应用型人才定位,并以“基础较厚、宽专适度、注重应用、强化能力”的培养思路,结合国内高校专业建设特色,强化“懂技术、会经营、善服务”核心竞争力培养,不断优化总结确定了适合我校发展的汽车服务工程“4341”人才培养定位,人才培养效果明显。     

体育教学促进学生竞争能力的实验研究

采用问卷调查法、实验法、数理统计法等进行体育教学促进学生竞争能力的实验研究。结果显示,实验前,实验班与对照班竞争能力(P>0.05)差异无显著意义。实验中,实验班施加培养竞争能力的因素,对照班正常教学。实验一年后,实验班学生竞争能力高于对照班学生(P<0.01)差异有非常显著意义。