| 组织工程修复软骨基质蛋白聚糖代谢的初步探讨 |
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| 引用本文： | 杨波,曹峻岭,张安,陈静宏,张增铁,付强,刘富强,陆敏灵,刘家远.组织工程修复软骨基质蛋白聚糖代谢的初步探讨[J].西安交通大学学报(医学版),2010,31(1). |
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| 作者姓名： | 杨波 曹峻岭 张安 陈静宏 张增铁 付强 刘富强 陆敏灵 刘家远 |
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| 作者单位： | 1. 西安交通大学医学院第一附属医院康复医学中心理疗科,陕西西安,710061
2. 西安交通大学医学院地方病研究所,环境与疾病相关基因教育部重点实验室,陕西西安,710061 |
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| 摘 要： | 目的 检测软骨修复组织中蛋白聚糖相关代谢指标,初步探讨在软骨修复过程中基质蛋白聚糖的代谢变化以及基质金属蛋白酶(matrix metalloproteinases, MMPs)和蛋白聚糖酶(aggrcanases)的作用.方法 松质骨骨基质明胶(bone matrix gelatin, BMG)复合同种异体软骨细胞构建组织工程化软骨,体内植入修复兔膝关节骨软骨缺损.术后6个月取材检测蛋白聚糖合成表位3-B-3(-)、MMPs、MMPs裂解表位BC-4 以及aggrcanases裂解表位BC-13的表达情况.结果 在修复组织中,蛋白聚糖合成表位3-B-3(-)表达增加,MMPs及其裂解表位BC-4表达减低,而aggrcanases裂解表位BC-13表达阴性.结论 利用蛋白聚糖合成表位3-B-3(-)、MMPs、BC-4、BC-13的表达情况可以初步了解修复软骨组织中蛋白聚糖的代谢变化,修复软骨组织蛋白聚糖的合成大于分解,MMPs在兔关节修复软骨基质蛋白聚糖的基础代谢和重塑中具有重要作用.
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| 关 键 词： | 组织工程 修复 软骨基质 蛋白聚糖 基质代谢 |
Research on matrix proteoglycan turnover of tissue engineering repaired cartilage |
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| YANG Bo,CAO Jun-ling,ZHANG An,CHEN Jing-hong,ZHANG Zeng-tie,FU Qiang,LIU Fu-qiang,LU Min-ling,LIU Jia-yuan.Research on matrix proteoglycan turnover of tissue engineering repaired cartilage[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(1). |
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| Authors: | YANG Bo CAO Jun-ling ZHANG An CHEN Jing-hong ZHANG Zeng-tie FU Qiang LIU Fu-qiang LU Min-ling LIU Jia-yuan |
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| Institution: | YANG Bo1,CAO Jun-ling2,ZHANG An2,CHEN Jing-hong2,ZHANG Zeng-tie2,FU Qiang2,LIU Fu-qiang2,LU Min-ling2,LIU Jia-yuan2(1.Department of Rehabilitation Medicine , Physiotherapy,the First Affiliated Hospital,Medical School of Xi\'an Jiaotong University,Xi\'an 710061,2.Institute of Endemic Diseases,Key Laboratory of Environment , Genes Related toDiseases of Ministry of Education,Xi\'an Jiaotong University,China) |
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| Abstract: | Objective To examine matrix proteoglycan metabolic markers and probe into the turnover of matrix proteoglycan and enzyme-mediated role of matrix metalloproteinases(MMPs) and aggrecanases in reparative tissues with tissue engineering cartilage.Methods Tissue-engineered cartilage was constructed by cancellous bone matrix gelatin(BMG) with allogeneic chondrocytes in vitro for 2 weeks,then implanted to repair osteochondral defects of rabbit knee joint.Samples were obtained 6 months later to explore the expressi... |
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| Keywords: | tissue engineering repair cartilage matrix proteoglycan matrix turnover |
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