| 谷氨酸受体-6在海人藻酸致痫大鼠发病机制中的作用 |
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| 引用本文： | 刘晓梅,孙伟,李小翠,孙亚峰,汤仁仙,裴冬生,张光毅.谷氨酸受体-6在海人藻酸致痫大鼠发病机制中的作用[J].西安交通大学学报(医学版),2010,31(2). |
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| 作者姓名： | 刘晓梅 孙伟 李小翠 孙亚峰 汤仁仙 裴冬生 张光毅 |
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| 作者单位： | 1. 徐州医学院基础学院
2. 徐州医学院肿瘤生物治疗重点实验室,江苏徐州,221004 |
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| 基金项目： | 国家自然科学基金资助项目，江苏省自然科学基金资助项目(No.BK2006536和No.BK2006035)Supported by the National Natural Science Foundation of China，the Natural Science Foundation of Jiangsu Province |
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| 摘 要： | 目的 研究谷氨酸受体-6(GluR6)在癫痫发病中的分子机制.方法 采用海人藻酸(KA)腹腔注射SD大鼠,用免疫沉淀、免疫印迹的方法检测在KA注射后不同时间点海马CA3区GluR6·PSD95·MLK3信号模块的组装.同时使用免疫组化和免疫印迹的方法观察小肽Tat-GluR6-9c对MLK3磷酸化水平的影响.结果 KA诱导了大鼠海马CA3区GluR6·PSD95·MLK3信号模块的组装,在KA刺激6h和12h达到结合高峰,1d后开始降低;而Tat-GluR6-9c抑制了KA注射6h MLK3的激活(P<0.05).结论 KA受体GluR6通过GluR6·PSD95·MLK3信号模块,激活了MLK3,参与了KA诱导的脑损伤.
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| 关 键 词： | 海人藻酸 癫痫 谷氨酸受体-6 |
Role of glutamate receptor-6 in kainate-induced epilepsy in rats |
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| LIU Xiao-mei,SUN Wei,LI Xiao-cui,SUN Ya-feng,TANG Ren-xian,PEI Dong-sheng,ZHANG Guang-yi.Role of glutamate receptor-6 in kainate-induced epilepsy in rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2010,31(2). |
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| Authors: | LIU Xiao-mei SUN Wei LI Xiao-cui SUN Ya-feng TANG Ren-xian PEI Dong-sheng ZHANG Guang-yi |
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| Institution: | LIU Xiao-mei1,SUN Wei1,LI Xiao-cui1,SUN Ya-feng1,TANG Ren-xian1,PEI Dong-sheng2,ZHANG Guang-yi1(1.School of Basic Medical Science,2.Laboratory of BiologicalCancer Therapy,Xuzhou Medical College,Xuzhou 221004,China) |
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| Abstract: | Objective To study the molecular mechanism of glutamate receptor-6(GluR6) in the pathogenesis of epilepsy.Methods Seizure model of SD rats was induced by intraperitoneal injection of kainate(KA).Immunoprecipitation and immunoblotting were performed to examine the interactions of GluR6 and MLK3 with PSD95 at various time points after KA injection.The effect of Tat-GluR6-9c on the MLK3 phospharylation induced by kainate was observed with immunoblotting and immunohistochemistry.Results The assembly of GluR6 an... |
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| Keywords: | MLK3 kainate epilepsy glutamate receptor-6 (GluR6) MLK3 |
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