谷氨酸介导的大脑皮层神经细胞凋亡

目的　观察相对低浓度谷氨酸 (Glutamate ,Glu)对培养皮层神经细胞的兴奋毒性作用。方法　以 30 μmol/L处理培养第 7天神经细胞 30min ,应用吖啶橙染色荧光显微镜观察及流式细胞仪定量分析法 ,分别观察Glu作用后细胞凋亡形态特征及不同时间点 ( 6h ,1 2h ,1 8h ,2 4h)凋亡细胞所占比例。结果　Glu作用后 6h即有凋亡细胞的出现 ,1 2h后渐增多 ,以 1 8h达到高峰 ,为 1 2 %。结论　相对低浓度Glu可诱导大脑皮层神经细胞凋亡。

The cerebral cortical neuronal apoptosis glutamate-induced

Objective To investigate exicitotoxicity of relative low concentration glutamate on the cultured cortical neurons. Methods The 7?d cultures were incubated with 30?μmol/L glutamate for 30?min. The apoptotic neurons were observed by fluorescence staining and FCM at different times (6?h,12?h,18?h,24?h).Results The apoptotic neuron appeared in 6?h,increased gradually in 12?h and peaked in 18?h (12%) after glutamate exposure.Conclusion The relative low concentration of glutamate may mediate apoptosis of the cultured cerebral cortical neurons.