电针对慢性癫痫大鼠海马新生神经元生长抑素表达的影响

目的 探讨电针对慢性癫痫大鼠海马新生神经元生长抑素(SS)表达的影响.方法 应用免疫荧光双标记和激光共聚焦显微镜观察了电针慢性癫痫大鼠督脉穴位"大椎"与"百会"后海马新生神经元生长抑素的表达情况.结果 海马新生神经元有SS的表达,且电针后的癫痫大鼠表达比未电针的癫痫大鼠减少,差异有统计学意义(P＜O.05).结论 电针可抑制海马新生神经元SS的表达,而SS有致痫作用.由此推测电针的抑痫机制可能是通过海马新生神经元SS的表达而实现的.

Influence of electroacupuncture on somatostatin expression of newborn neurons in the hippocampus of chronic epilepsy rats

Objective To explore influence of electroacupuncture on somatostatin expression of newborn neurons in the hippocampus of chronic epilepsy rats.Methods The double-label immunofluorescence with confocal microscope was used to observe somatostatin expression of newborn neurons in the hippocampus of chronic epilepsy rats after electroacupuncture stimulation of Dazhui (GV14)' and Baihui (GV20)' in DuMai.Results Somatostatin expression of newborn neurons was found in the hippocampus.Moreover,in somatostatin expression of newborn neurons,epileptic rats with electroacupuncture had less than epileptic rats in the hippocampus.The former had an obviously significant difference compared with the latter (P<0.05).Conclusion Somatostatin is involved in proconvulsion.Electroacupuncture inhibits somatostatin expression of newborn neurons in the hippocampus of chronic epilepsy rats.Electroacupuncture inhibits epileptogenesis by decreasing somatostatin colocalized with newborn neurons in hippocampus.