| 海马CA3区损伤对齿状回颗粒细胞下层新生细胞向颗粒层迁移的影响 |
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| 引用本文： | 刘建新,刘勇.海马CA3区损伤对齿状回颗粒细胞下层新生细胞向颗粒层迁移的影响[J].西安交通大学学报(医学版),2011,32(5):550-552,556. |
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| 作者姓名： | 刘建新 刘勇 |
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| 作者单位： | 西安交通大学医学院神经生物学研究所,环境与疾病相关基因教育部重点实验室,陕西西安710061 |
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| 基金项目： | 高等学校博士学科点专项科研基金(新教师类)资助项目 |
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| 摘 要： | 目的 明确海马CA3区损伤对齿状回颗粒细胞下层(SGZ)新生细胞向颗粒细胞层迁移的影响.方法 BrdU标记齿状回SGZ神经干细胞,Ibotenic acid或同体积生理盐水海马CA3区立体定位注射,4周后BrdU+ NeuN免疫荧光染色观察分析迁移到齿状回颗粒细胞层的新生细胞的数量和比例,分别采用t-test和两因素方...
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| 关 键 词： | 海马 神经再生 齿状回颗粒细胞下层(SGZ) CA3区 |
The effect of CA3 lesion on the migration of SGZ new-born cells to the granule cell layer in dentate gyrus of adult rats |
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| LIU Jian-xin,LIU Yong.The effect of CA3 lesion on the migration of SGZ new-born cells to the granule cell layer in dentate gyrus of adult rats[J].Journal of Xi‘an Jiaotong University:Medical Sciences,2011,32(5):550-552,556. |
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| Authors: | LIU Jian-xin LIU Yong |
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| Institution: | LIU Jian-xin,LIU Yong(Institute of Neurobiology,Key Laboratory of Environment and Genes Related to Diseases ofEducation Ministry,Medical School of Xi'an Jiaotong University,Xi'an 710061,China) |
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| Abstract: | Objective To explore the effect of CA3 lesion in controlling the migration of SGZ new-born cells to the granule cell layer in dentate gyrus of adult rats.Methods After intraperitoneal injection of BrdU,Ibotenic acid or saline was injected into the unilateral CA3 area of hippocampus using stereotaxic techniques.The animals were killed on day 32,followed by BrdU+NeuN double labeling.The number and ratio of new-born cells that had reached the granule cell layer in dentate gyrus were observed,and the data were ... |
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| Keywords: | hippocampus neurogenesis subgranular zone of dentate gyrus CA3 area |
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