一种血管生物反应器相关参数的量化分析

为了模拟血管的在体环境,由北京航空航天大学与四川大学联合为西南交通大学研制了血管生物反应器,采用流体力学理论与正交实验极差分析的方法对生物反应器的剪切力、层流状态及脉压差调节等性能指标进行了量化分析.结果表明:该套系统针对不同半径的血管,可提供的最小剪切力为0.08 Pa,最大剪切力为70.51 Pa;为保证灌流液为层流状态,在灌流液粘度系数、流体密度确定的前提下,培养腔入口端输入管长度为18 cm时,装置提供的5种管径的最大流量均不可超过4.36 cm3/s;脉动发生器的行程对收缩压与舒张压的压差影响最大,对流量和频率的影响次之,对压力的影响最小. 

Quantitative Analysis of Parameters of Vascular Bioreactor

A vascular bioreactor was designed and constructed by Beihang University and Sichuan University for Southwest Jiaotong University for simulating in vivo environments.The performance indicators of the bioreactor including shearing stress,the state of laminar flow,and pulse pressure were analyzed quantitatively through theoretical derivation of hydromechanics and orthogonal experiment range analysis.The results show that for blood vessels of different diameters,the bioreactor could provide shearing stress ranging from 0.08 to 70.51 Pa.In order to maintain a laminar flow condition of the perfusate,the maximum flow rate in the tubes of five kinds of diameters should not exceed 4.36 cm3/s when the inlet tube length of the culture chamber was 18 cm,and the viscosity coefficient and fluid density of the perfusate were fixed.The operating distance of the pulsation generator had a bigger effect on the difference between systolic and diastolic blood pressures than the flow and frequency,while the effect of pressure was the smallest.