MULTIPLE ENHANCER ELEMENTS MEDIATE THE INDUCTION OF C-FOS BY ANGIOTENSIN Ⅱ

ＭＵＬＴＩＰＬＥＥＮＨＡＮＣＥＲＥＬＥＭＥＮＴＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＢＹＡＮＧＩＯＴＥＮＳＩＮⅡＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．...     

MULTIPLE TRANS-ACTING FACTORS MEDIATE THE INDUCTION OF C-FOS IN VASCULAR SMO

ＭＵＬＴＩＰＬＥＴＲＡＮＳ－ＡＣＴＩＮＧＦＡＣＴＯＲＳＭＥＤＩＡＴＥＴＨＥＩＮＤＵＣＴＩＯＮＯＦＣ－ＦＯＳＩＮＶＡＳＣＵＬＡＲＳＭＯＯＴＨＭＵＳＣＬＥＣＥＬＬＳＣｈｅｎＹａｎｑｕｎ；ＢａｒｔｅｋＲｙｄｚｅｗｓｋｉ，ＡｌｌｅｎＪ．Ｎａｆｔｉｌａｎ（Ｄｅ...     

DYNAMIC CHANGES IN Giα2 LEVELS IN THE RAT HEART ASSOCIATED WITH IMPAIRED CARDIAC FUNCTION FOLLOWING ACUTE MYOCARDIAL INFA RCT

ＤＹＮＡＭＩＣＣＨＡＮＧＥＳＩＮＧｉα２ＬＥＶＥＬＳＩＮＴＨＥＲＡＴＨＥＡＲＴＡＳＳＯＣＩＡＴＥＤＷＩＴＨＩＭＰＡＩＲＥＤＣＡＲＤＩＡＣＦＵＮＣＴＩＯＮＦＯＬＬＯＷＩＮＧＡＣＵＴＥＭＹＯＣＡＲＤＩＡＬＩＮＦＡＲＣＴＩＯＮＳｋｉＢｉｎｇ；Ｊ．Ｅ．Ｈｅａ...     

LEISHMANIA PROMASTIGOTES EVADE INTERLEUKIN 12 INDUCTION BY MACROPHAGES AND STIMULATE A BROAD RANGE OF CYTOKINES FROM CD_（4）~（＋

ＬＥＩＳＨＭＡＮＩＡＰＲＯＭＡＳＴＩＧＯＴＥＳＥＶＡＤＥＩＮＴＥＲＬＥＵＫＩＮ１２ＩＮＤＵＣＴＩＯＮＢＹＭＡＣＲＯＰＨＡＧＥＳＡＮＤＳＴＩＭＵＬＡＴＥＡＢＲＯＡＤ　ＲＡＮＧＥＯＦＣＹＴＯＫＩＮＥＳＦＲＯＭ　ＣＤ_（４）~（＋）ＴＣＥＬＬＳＤＵＲＩＮＧＩ...     

REGULATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASEPKR AND2＇5＇ OLIGOADENYLATE SYNTHETASE BY A CATALYTICALLY INACTIVE PKR M

ＲＥＧＵＬＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＡＮＤ２＇５＇ＯＬＩＧＯＡＤＥＮＹＬＡＴＥＳＹＮＴＨＥＴＡＳＥＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＰＫＲ　ＭＵＴＡＮＴＴＨＲＯＵＧＨ　Ｃ...     

DIETARY MAGNESIUM DEFICIENCY INCREASES LEVELS AND FUNCTION OF Giα IN THE RAT HEART AFTER MYOCARDIAL INFARCTION

ＤＩＥＴＡＲＹＭＡＧＮＥＳＩＵＭＤＥＦＩＣＩＥＮＣＹＩＮＣＲＥＡＳＥＳＬＥＶＥＬＳＡＮＤＦＵＮＣＴＩＯＮＯＦＧｉαＩＮＴＨＥＲＡＴＨＥＡＲＴＡＦＴＥＲＭＹＯＣＡＲＤＩＡＬＩＮＦＡＲＣＴＩＯＮＳｈｉＢｉｎｇ；Ｊ．Ｅ．Ｈｅａｖｎｅｒ，Ｌ．Ｍ．Ｂｏｙｌａｎ...     

PROTECTIVE EFFECT OF 3，6－DIMETHAMIDODIBENZOPYRIODONIUM CITRATE ON CALCIUM PARADOX IN THE ISOLATED GUINEA PIG HEART

ＰＲＯＴＥＣＴＩＶＥＥＦＦＥＣＴＯＦ３，６－ＤＩＭＥＴＨＡＭＩＤＯＤＩＢＥＮＺＯＰＹＲＩＯＤＯＮＩＵＭＣＩＴＲＡＴＥＯＮＣＡＬＣＩＵＭＰＡＲＡＤＯＸＩＮＴＨＥＩＳＯＬＡＴＥＤＧＵＩＮＥＡＰＩＧＨＥＡＲＴ（孙良起）（袁素芬）（马宏锐）（邱培伦）ＳｕｎＬ...     

REVERSAL OF THE DOUBLE－STRANDED－RNA－INDUCED INHIBITION OF PROTEIN SYNTHESIS BY A CATALYTICALLY INACTIVE MUTANT OF THE PROTEIN

ＲＥＶＥＲＳＡＬＯＦＴＨＥＤＯＵＢＬＥ－ＳＴＲＡＮＤＥＤ－ＲＮＡ－ＩＮＤＵＣＥＤＩＮＨＩＢＩＴＩＯＮＯＦＰＲＯＴＥＩＮＳＹＮＴＨＥＳＩＳＢＹＡＣＡＴＡＬＹＴＩＣＡＬＬＹＩＮＡＣＴＩＶＥＭＵＴＡＮＴＯＦＴＨＥＰＲＯＴＥＩＮ　ＫＩＮＡＳＥＰＫＲＴｙｓｏｎ...     

THE LA ANTIGEN INHIBITS THE ACTIVATION OF THE INTERFERON－INDUCIBLE PROTEIN KINASE PKR BY SEQUESTERING AND UNWINDING DOUBLE－ST

ＴＨＥＬＡＡＮＴＩＧＥＮＩＮＨＩＢＩＴＳＴＨＥＡＣＴＩＶＡＴＩＯＮＯＦＴＨＥＩＮＴＥＲＦＥＲＯＮ－ＩＮＤＵＣＩＢＬＥＰＲＯＴＥＩＮＫＩＮＡＳＥＰＫＲＢＹＳＥＱＵＥＳＴＥＲＩＮＧＡＮＤＵＮＷＩＮＤＩＮＧＤＯＵＢＬＥ－ＳＴＲＡＮＤＥＤＲＮＡＸｉａｏＱｉｕ...     

TARGETED ACTIVATION OF CD_（8）~（＋） CELLS AND INFECTION OF β_2－MICROGLOBULIN－DEFICIENT MICE FAIL TO CONFIRM A PRIMARY PROTECTIVE

ＴＡＲＧＥＴＥＤＡＣＴＩＶＡＴＩＯＮＯＦＣＤ_（８）~（＋）ＣＥＬＬＳＡＮＤＩＮＦＥＣＴＩＯＮＯＦβ_２－ＭＩＣＲＯＧＬＯＢＵＬＩＮ－ＤＥＦＩＣＩＥＮＴＭＩＣＥ　ＦＡＩＬＴＯＣＯＮＦＩＲＭＡＰＲＩＭＡＲＹＰＲＯＴＥＣＴＩＶＥＲＯＬＥＦＯＲＣＤ（８）~（＋...     

大白鼠脑干脊髓投射——HRP法研究

本研究用大白鼠12只,于脊髓颈膨大 (8例) 和腰膨大 (4例) 处背中线右侧,3点分别注射50％HRP水溶液,总量为0.6—1.5微升。在脑干内看到HRP标记细胞,分布如下: 1.延髓:巨细胞网状核,旁正中网状核,外侧网状核,中缝苍白核,中缝隐核,三叉神经脊束核,孤束核和下橄榄核等。 2.脑桥:桥吻侧网状核,桥尾侧网状核、中缝大核,前庭神经核、兰斑核、三叉神经主核。 3.中脑:中缝背核,中线核,艾一维氏 (E—M核) 核,被盖腹侧核,楔核、上、下丘核,红核和黑质。     

衰老大鼠脑干中缝核群酪氨酸羟化酶样神经元的变化——免疫金银染色法和图像定量分析研究

本文结合免疫金银染色法和图像定量分析,观察幼年(2个月)、中年(10个月)和老年(24个月)大鼠脑干中缝核群酪氨酸羟化酶祥(THL)神经元的衰老变化,结果表明:THL神经元主要分布于中缝背核、中央上核和嘴侧线形核內;THL神经无从中年即开始丧失,到老年,其数量几乎减少1/3;图像分析表明,THL神经元的截面积随增龄而增大,但形态观察发现,老年组的阳性神经元的胞体萎缩,边缘皱折而呈不规则状,突起缩短、锐化或扭曲;THL神经元的灰度值随增龄而升高,说明儿茶酚胺的含量和转换率在衰老进程中是逐渐降低的。     

大白鼠海马传入纤维的来源——HRP法研究

本文用25只雌性成年大白鼠,体重150～250克,将33％的HRP(SigmaⅥ,R.Z=2.9～3.1)分别注射于右侧背海马或腹海马,在下列各核区发现HRP标记细胞。 1.本侧:内侧隔核、上乳头体核、下乳头丘脑束核、中缝背核、正中中缝核、蓝斑核、内嗅皮层、斜角带和上丘等。 2.对侧:海马的CA3、CA4区和蓝斑核。     

衰老大鼠脑干中缝核群P-物质样神经元的变化——免疫金银染色法和图象定量分析研究

结合免疫金银染色(IGSS)法和图象定量分析,观察了不同年龄大鼠脑干中缝核群P-物质(SP)样神经元的衰老变化。结果表明:在中缝核群内,SP 样神经元主要分布于中缝苍白核、中缝隐核、中缝大核和中缝背核内。中缝背核内的SP样神经元从中年即开始丧失,而其它核团的神经元到老年才开始减少(P<0.01)。从幼年到中年,各核团的SP 样神经元的截面积几乎增大一倍;但到老年,仅于中缝苍白核内的神经元还在继续增大(P<0.05),而其它核团没有明显变化。各核团的SP 样神经元的灰度值均呈增龄性升高,提示神经元的SP 含量和活性在衰老进程中逐渐降低。老年组SP 样神经元的形态改变呈现为胞体肿胀,边缘不规整,免疫反应减弱,染色浅淡。     

对电针反应的大鼠中缝大核神经元的特性

在大鼠中缝大核中记录到100个神经元的单位活动,被分类为伤害兴奋型(59个),伤害抑制型(30个),非伤害感受型(5个)和其它类型神经元(6个)。试验了70个神经元对电针的反应,表明电针可激活各类神经元的活动,使大部分伤害兴奋神经元(69.77%)兴奋,伤害抑制神经元(68.42%)抑制。中脑导水管周围灰质有下行纤维投射到中缝大核中,加强对电针反应神经元的活动。全身应用吗啡对电针反应神经元呈现兴奋、抑制和无作用3种型式,但同电针反应型式之间无明显相关关系,表明中缝大核在针刺镇痛与吗啡镇痛中的作用只是部分重合而不是完全一致。     

THE EFFECT OF LIGUSTRAZINE ON NEUROGENESIS IN CORTEX AFTER FOCAL CEREBRAL ISCHEMIA IN RATS

Objective To explore the effect of Ligustrazine on neurogenesis in cortex after focal cerebral ischemia in rats. Methods Focal cerebral ischemia was induced by left middle cerebral artery occlusion with a suture. Two hours later, injection of Ligustrazine （80 mg/kg, 1 time/d） was performed peritoneally. Four hours after the ischemia, 5-bromodeoxyuridine （BrdU） （50 mg/kg, 1 time/d） was injected peritoneally. At 7 d, 14 d and 21 d after ischemia, BrdU positive cells in the cortex were observed by cal staining. Results In ischemic model group, at 7 day, sparsely-distributed BrdU positive cells were observed in the Ⅱ-- Ⅵ layers of the ipsilateral cortex, with a bandlike distribution in ischemic penumbra. With the prolongation of ischemia, the number of BrdU positive cells increased. In Ligustrazine group, BrdU positive cells were also observed in theⅡ-- Ⅵ layers of the cortex, with an intense distribution in ischemic penumbra. The numbers of BrdU positive cells at 7 d, 14 d and 21 d were more than those in ischemic model group respectively. Conclusion Ligustrazine increases the proliferated cells in cortex after focal cerebral ischemia in rats. The results suggest that it may be useful for promoting self-repair after ischemia.     

灵长类运动辅助区的丘脑联系

目的　观察运动辅助区的丘脑起源。方法　采用束路示踪技术 ,将 2种逆行示踪剂分别注射到猴子运动辅助区的嘴侧部 (Pre SMA)和尾侧部 (SMA proper)。结果　丘脑腹前核的小细胞部 (VApc)、X区 (X)和背内侧核的外侧部 (MDl)发出纤维投射到Pre SMA。丘脑腹外侧核的嘴侧部 (VLo)、腹后外侧核的嘴侧部 (VPLo)、腹外侧核的尾侧部 (VLc)和背内侧核的腹侧部(MDv)则主要投射到SMA proper。 结论　运动辅助区是小脑和基底神经节环路的共同靶点 ,它们通过不同的丘脑核团影响Pre SMA和SMA proper的活动     

灵长类主要运动区和背侧运动前区的丘脑联系

目的　观察灵长类主要运动区和背侧运动前区的丘脑起源。方法　采用束路示踪技术 ,将 2种逆行示踪剂分别注射到猴子的主要运动区 (M 1)和背侧运动前区 (PMd)。结果　丘脑腹外核嘴侧部 (VLo) ,腹内侧核(VM ) ,腹后外侧核嘴侧部 (VPLo) ,腹外侧核尾侧部 (VLc) ,中央中核 (CM ) ,中央外侧核 (CL)和腹后内侧核(VPM )均有纤维投射到M 1。丘脑腹外侧核嘴侧部 (VLo) ,腹后外侧核的嘴侧部 (VPLo) ,腹外侧核的尾侧部(VLc)和背内侧核 (MD)则主要投射到背侧运动前区 (PMd)。结论　M1和PMd具有不同的丘脑起源 ,这种起源既有重叠也有分离 ;小脑和基底神经节通过不同的丘脑核团影响运动区皮层的活动     

高糖对MMP-2与TIMP-2的表达及血管平滑肌细胞增殖的影响

目的探讨高糖作用下大鼠胸主动脉平滑肌细胞基质金属蛋白酶-2(MMP-2)及其组织抑制剂-2(TIMP-2)的表达情况及高糖对血管平滑肌细胞(VSMCs)增殖的影响。方法大鼠胸主动脉平滑肌细胞培养及传代后,分为正常浓度葡萄糖组(NG组,5.5mmol/L葡萄糖)、甘露醇高渗对照组(5.5mmol/L葡萄糖+19.5mmol/L甘露醇)、高浓度葡萄糖组(HG组,25mmol/L葡萄糖)和GM6001组(25mmol/L葡萄糖+5μmol/L GM6001),在4组不同的培养环境下分别培养72h后,用RT-PCR技术检测MMP-2与TIMP-2的表达情况,同时用MTT比色法检测4种不同培养环境下VSMCs的增殖情况。结果 HG组与NG组相比,MMP-2与TIMP-2mRNA的表达增强,差异具有统计学意义(P<0.01),甘露醇高渗对照组、GM6001组与NG组相比,MMP-2与TIMP-2mRNA的表达差异无统计学意义(P>0.05);HG组与NG组相比,MMP-2与TIMP-2 mRNA的相对表达量的比值显著增加,差异具有统计学意义(P<0.01),甘露醇高渗对照组、GM6001组与NG组相比差异无统计学意义(P>0.05);HG组各时间点细胞增殖与NG组相比差异具有统计学意义(P<0.05),而GM6001组、甘露醇高渗对照组各时间点的细胞增殖与NG组相比差异无统计学意义(P>0.05)。结论高浓度葡萄糖促进血管平滑肌细胞增殖,这可能是通过MMP-2与TIMP-2表达失衡介导的。     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.