新疆四县农村3岁以下儿童营养状况分析

目的　了解新疆维吾尔族自治区 (简称新疆 )农村 3岁以下儿童的营养状况 ,为针对性开展干预提供决策依据。方法　对新疆 4县进行了随机抽样横断面调查 ,以身高和体重作为衡量儿童营养状况的指标。结果　该地区 3岁以下儿童生长迟缓 (HAZ <- 2 )、低体重 (WAZ <- 2 )和消瘦 (WHZ <- 2 )的总患病率分别为 2 4.3 %、2 3 .1%和 9.1% ;维吾尔族高于汉族 ;经济差的县高于经济好的县。与NCHS/WHO标准分布比较 ,新疆儿童HAZ和WAZ的分布显著左移。结论　新疆农村 3岁以下儿童存在着较严重的营养问题。应积极开展营养健康教育 ,加强儿童常见病的防治。在重点干预的同时 ,还要注意改善整体儿童的营养状况     

TRAIL在食道鳞癌及癌旁组织中的表达

目的　研究人肿瘤坏死因子相关凋亡诱导配体 (TNF relatedapoptosisinducingligand ,TRAIL)在食道鳞癌及癌旁组织中的表达及意义。方法　采用免疫组化S -P法 ,检测 42例食道鳞状细胞癌及其癌旁组织中TRAIL蛋白表达水平。结果　TRAIL在正常食道粘膜上皮、单纯增生、不典型增生、鳞癌组织中其阳性表达呈递减趋势 (P <0 .0 1) ;在Ⅰ、Ⅱ级鳞癌明显高于Ⅲ级和未分化鳞癌 (P <0 .0 1)。而在早期鳞癌和晚期鳞癌中的表达无差异 (P >0 .0 5 )。伴有淋巴结转移组和无转移组之间无差异 (P >0 .0 5 )。结论　TRAIL的表达可能与食道鳞癌的发生、发展密切相关 ;与食道鳞癌的分级呈负相关 ;与癌组织浸润深度和淋巴结转移无关。     

GUSTATORY RESPONSES OF NEURONS IN THE PARABRACHIAL NUCLEUS OF RATS

Ａｎｕｍｂｅｒｏｆａｎａｔｏｍｉｃａｌ,ｅｌｅｃｔｒｏｐｈｙｓｉｏｌｏｇｉｃａｌａｎｄｂｅｈａｖｉｏｒａｌｅｘｐｅｒｉｍｅｎｔｓｈａｖｅｓｈｏｗｎｔｈａｔｇｕｓｔａｔｏｒｙｉｎｆｏｒｍａｔｉｏｎｉｎｔｈｅｎｕｃｌｅｕｓｏｆｔｈｅｓｏｌｉｔａｒｙｔｒａｃｔ(ＮＴＳ) ,ｗｈｉｃｈｉｓｃｏｎｖｅｙｅｄｆｒｏｍｔｏｎｇｕｅｖｉａｔｈｅｃｒａｎｉａｌｎｅｒｖｅｓ (ｆａｃｉａｌ,ｇｌｏｓｓｏｐｈａｒｙｎｇｅａｌ,ａｎｄｖａｇｕｓ) ,ｒｅａｃｈｅｓａｃｏｎｆｉｎｅｄ ｐａｒｔｏｆｔｈｅ ｐａｒａｂｒａｃｈｉａｌｎｕｃ…     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

单纯疱疹病毒2型gG-2"独特区"基因的原核表达及其血清学诊断价值

目的在大肠杆菌中表达HSV-2的gG-2"独特区"基因(gG-2T)并对其免疫学特性进行初步研究。方法克隆HSV-2的gG-2T,并在大肠杆菌中表达gG-2T融合蛋白。通过Western blot和间接ELISA鉴定融合蛋白的活性。结果通过原核系统表达的gG-2T融合蛋白可以与GST多克隆抗体结合并在46 ku附近出现特异性结合条带。并且能够与确诊的HSV-2患者血清反应,而不与HSV-1患者血清和正常人血清反应。结论获得了HSV-2 gG-2T融合蛋白,它可以与GST多克隆抗体特异性结合并且可以同HSV-2型患者血清特异性反应;获得的gG-2T蛋白为研究以gG-2蛋白作为靶区域的诊断试剂盒和疫苗研发打下实验基础。     

PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

BUILDING OF A SIMPLE MODEL OF AUTO-OLT IN DOGS AND ITS SIGNIFICANCE

ＢＵＩＬＤＩＮＧＯＦＡＳＩＭＰＬＥ　ＭＯＤＥＬＯＦＡＵＴＯ－ＯＬＴＩＮＤＯＧＳ　ＡＮＤＩＴＳＳＩＧＮＩＦＩＣＡＮＣＥＫａｎｇＹａ＇ａｎ；，ＬｉＹｉｍｉｎｇ，ＪｉＺｏｎｇｚｈｅｎｇ；，ＱｉｎＺｈａｏｙｉｎ;（ＤｅｐａｒｔｍｅｎｔｏｆＧｅｎｅｒａｌＳｕｒ?..     

基于小波域隐马尔可夫树模型的多光谱遥感影像纹理分割技术研究

遥感图像纹理信息丰富，需要进行有效的纹理特征描述．小波域隐马尔可夫树（HMT）模型提供了一种有效的纹理特征提取方法．简述了HMT模型及分割思想，并在此基础上针对多光谱遥感图像，探索更好的纹理描述方法．通过适当的数学变换，得到多光谱遥感影像在不同角度下的描述：H—S—I分量和最大主成分分量．研究比较各分量特性并应用HMT模型，得到不同分量下纹理分割结果．实验表明，利用多光谱信息可有效地提高分割质量。     

生成模型在中心城市的应用实践

本文从城市的区域交通节点考虑,把城市分为中心城市、资源型城市和一般城市.以中心城市土地利用特性和现状人口为基础,采用曲线估计的方法建立了交通生成预测模型.利用辽宁省锦州市、盘锦市和辽阳市的交通数据,对模型进行了验证,结果表明模型能够较好反映规划年城市土地利用特征变化所带来的各种交通需求特性的差异.     

西安汉族、西藏珞巴族DXS6799和HPRTB位点的遗传多态性

目的调查X染色体STR位点DXS6799、HPRTB在西安汉族、西藏珞巴族的遗传多态性。方法应用PCR和聚丙烯酰胺凝胶电泳及银染技术进行基因分型。结果西安汉族DXS6799检出6个等位基因,基因频率在0.0160～0.4840之间,女性和男性的个体识别率分别为0.8516、0.6157;HPRTB位点检出8个等位基因,等位基因频率在0.011～0.290之间,女性和男性的个体识别率分别为0.7974、0.7039。西藏珞巴族DXS6799检出5个等位基因,基因频率为0.0222～0.6074之间,女性和男性的个体识别率分别为0.7684、0.5805;HPRTB位点检出5个等位基因,等位基因频率在0.0420～0.3866之间,女性和男性的个体识别率分别为0.8661、0.7029。西安汉族、西藏珞巴族女性的基因型分布均符合Hardyweinberg平衡。结论DXS6799和HPRTB有较高的个体识别率和非父排除率,在个体识别和亲权鉴定中有应用价值。