矢量型电子河道图岸线提取方法研究

为快速准确获取河道岸形线的数据,生成缺乏岸线数据资料的河道矢量型轮廓图,提出一种通过处理雷达图像获取矢量型电子河道图岸形线数据的方法。采用了数学形态学方法实施对雷达图像处理,主要的处理过程包括:雷达图像灰度处理,灰度雷达图像转变为二值图像,二值雷达图像的滤波处理,在以上处理过程的基础上构造了数学形态学结构元素,实施对河道岸线提取,并矢量化处理了离散点。在二值化过程中提出新的方法,针对雷达图像的特点,依据航路规则设计了特殊的滤波过程,提出了提取河道任意边缘的方法。     

浅析汽车维修新技术中发动机故障检测及维修技术

随着我国社会发展进步,汽车已经成为人民生活中不可或缺的一部分,而汽车运行过程中难免会发生故障,其中发动机故障的影响尤为严重。从简述汽车维修新技术的重要作用入手,进一步分析汽车发动机故障的检测方法,探究汽车维修新技术在发动机异响、发动机油样、发动机启停及发动机冷却系统故障检测和维修的途径。     

反坡斜井突涌水施工技术

通过对锦屏山隧道辅引3#施工支洞反坡施工,介绍反坡斜井突涌水条件下施工技术,根据实例论述了以防为主、防排结合的反坡斜井突涌水隧道施工方法.     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

图像边界检测区域对比度模糊增强算法在轮廓提取中的运用

分析了图像边界检测的区域对比度模糊增强算法的特点，通过实验验证了该算法的正确性和可行性，并指出了算法的一些不足之处和待改进的地方，提出了一种适用轮廓提取的新广义算子。     

汽车维修中检测诊断技术的应用探讨

现如今汽车已经成为人们必不可少的代步工具,汽车的数量随之也越来越多,在汽车的行驶过程中,难免会出现故障之类的问题,因此汽车的维修工作也变的尤为重要,其维修的师傅必须具备丰富的工作经验以及理论知识,然后借助检测诊断技术来进行汽车的事故和问题故障的检测排查工作。本文围绕汽车的检测诊断技术以及该技术在汽车维修中的应用展开分析。     

基于STM32的蓄电池内阻测量系统设计

文章设计了一款以STM32微处理器为核心的蓄电池内阻测量系统,采用交流阻抗法对电池内阻进行测量。针对测量信号微弱,易淹没于噪声的特点,应用相关检测技术以检出有用信息。调试和对比实验数据表明,该系统可实现对蓄电池内阻的在线、快速、精准测量。     

Efficient multiple model particle filtering for joint traffic state estimation and incident detection

This article proposes an efficient multiple model particle filter (EMMPF) to solve the problems of traffic state estimation and incident detection, which requires significantly less computation time compared to existing multiple model nonlinear filters. To incorporate the on ramps and off ramps on the highway, junction solvers for a traffic flow model with incident dynamics are developed. The effectiveness of the proposed EMMPF is assessed using a benchmark hybrid state estimation problem, and using synthetic traffic data generated by a micro-simulation software. Then, the traffic estimation framework is implemented using field data collected on Interstate 880 in California. The results show the EMMPF is capable of estimating the traffic state and detecting incidents and requires an order of magnitude less computation time compared to existing algorithms, especially when the hybrid system has a large number of rare models.     

主动声纳的低截获性数字仿真研究

在现代海战中,舰艇面临着越来越严峻的敌方侦察声纳的威胁.低截获是一项非常诱人的技术.低截获声纳通过选择合适的发射信号,在保持探测能力的同时,降低被敌方侦察声纳截获的概率.采用数字仿真的形式,分析浅海侦察声纳信号能量的变化,研究主动声纳的低截获性.仿真结果表明在高斯噪声背景下,主动声纳实现低截获性相当困难.     

交通运输超限治理对策研究

由于交通运输量的不断增大以及运输行业的恶性竞争等原因，导致货车超载现象越来越普遍，并已成为急需解决的问题。探讨交通运输超限治理的对策，对于完善科学而系统的超限管理体系具有一定的借鉴意义。