焊接转向架构架仿真分析及试验研究

介绍了新型转向架结构组成和构架材料选择,并依据EN 13749-2011标准相关内容,对新研制转向架构架进行有限元分析和强度试验;利用Hypermesh软件对构架三维模型进行离散化处理,对其进行有限元分析;采用一种新构架试验方法,在疲劳试验台进行静强度试验和疲劳试验;通过在试验过程中施加标定工况和正常运营工况载荷,监控构架和试验工装的状态。仿真分析和试验结果表明,新型转向架构架主体结构能够满足相关标准要求。     

基于宽带接入服务器的分布式安全审计系统

用宽带接入服务器(BAS)作为分布式审计代理实现城域网(MAN)上的主动式安全审计系统．提出一种新的硬件包过滤机制(HPF)以适合系统的需要．BAS上的路由转发机制从“一次路由，多次交换”改为“一次审计，多次通过”．采用基于时间推理机的算法完成对UDP访问的审计．系统在试验网络中进行了测试，显示了很好的审计处理性能。     

Radar Emitter Signal Recognition Based on Complexity Features

Intra-pulse characteristics of different radar emitter signals reflect on signal waveform by way of changing frequency, phase and amplitude. A novel approach was proposed to extract complexity features of radar emitter signals in a wide range of signal-to-noise ratio ( SNR), and radial basis probability neural network (RBPNN) was used to recognize different radar emitter signals. Complexity features, including Lempel-Ziv complexity (LZC) and correlation dimension (CD), can measure the complexity and irregularity of signals, which mirrors the intra-pulse modulation laws of radar emitter signals. In an experiment, LZC and CD features of 10 typical radar emitter signals were extracted and RBPNN was applied to identify the 10 radar emitter signals. Simulation results show that the proposed approach is effective and has good application values because average accurate recognition rate is high when SNR varies in a wide range.     

In Vitro Model of Human Choroidal Neovascular

Choroidal capillary endothelia cell (CEC) plays a critical role in the development of choroidal neovascularization which is one of the major causes of blindness. An effective method for CEC cultivation was proposed. The isolation of human choroidal CECs using micro dissection followed by the use of superparamagnetic beads (Dynabeads) coated with the CD 31, which selectively binds to the endothelial cell surface. Cells bound to beads were isolated using a magnetic particle concentrator. The CECs were planted into type IV collagen coated 24 well plates. The results show that the primary cultured CEC is induced to tube formation in collagen IV coated environment, which can be presented as an in vitro model of choroidal neovascularization.     

Distributed Broker-Agent Architecture for Multimedia Communications Traversing NAT／Firewall in NGN

The forthcoming Next Generation Network (NGN) is an all IP network. Multimedia communications over IP networks are a type of bundled session communications, which cannot directly traverse Network Address Translations (NATs) and firewalls even in NGN. To solve the problem that the existing traversal methods are not suitable for service providers to set up a real system in NGN,a Distributed Broker-agent Architecture (DBA) is addressed. DBA is secure and realizable for service providers and enterprises because it is easy to set up and does not need to upgrade the existing devices like Firewalls, NATs or endpoint devices of subscribers.DBA is composed of two-layer distributed agents, the server proxies and the client agents, in which all multimedia communications use shared tunnels to carry signaling messages and media data between broker-agents, and the call signaling is encrypted over Security Socket Layer (SSL) to guarantee the security of calling. Moreover, the function model and multiplexed connection messages format of DBA are designed, which lays a basis for the protocol in the future NGN. In addition, a simple implementation based on H.323 verifyies the main function of traversing firewalls and NATs.     

脆性组氨酸三聚体蛋白和蛋白激酶Ca的表达及其与非小细胞肺癌侵袭、转移的相关性

目的 探讨脆性组氨酸三聚体（FHIT）蛋白和蛋白激酶Ca（cPKCa）在非小细胞肺癌（NSCLC）中的表达及其与NSCLC侵袭、转移的相关性。方法 采用免疫组化PicTure^TM通用型二步法，对41例NSCLC组织和11例肺良性病变组织行FHIT蛋白和cPKCn检测。结果 FHIT蛋白和cPKCa在NSCLC组织中的阳性表达率分别为48．8％和53．7％，在肺良性病变组织中的阳性表达率分别为90．9％和18．20％，均有显著性差异（P〈0．05）；COX比例风险模型生存分析结果显示，FHIT蛋白（P=0．020，RR=2．754）、cPKCa（P=0．022，RR=0．385）具有独立的预后意义。结论 FHIT蛋白和cPKCa表达与NSCLC的发展、侵袭及转移显著相关，可作为判断NSCLC临床预后的参考指标。     

基于频率和形状特征的脉冲重复间隔调制识别

根据雷达信号脉冲序列的特点，从雷达脉冲信号中提取频率特征和形状特征，构成二维特征向量，并用支持向量机设计多类别分类器，实现雷达信号PRI调制信号的自动识别．实验结果表明，对特征向量进行大幅度降维（从64维降到2维）后，既简化了分类器，又保持或提高了识别率和抗噪声性能．与原特征向量相比，对无噪样本的误识率从0．15％～0．25％降低到0．00％，对有噪样本的误识率从0．40％～1．30％降低到0．15％～0．93％．     

恶意主机上Mobile Agent安全解决方案

根据非对称加密原理提出了恶意主机上Mobile Agents（MA）的安全解决方案。在该方案中，只有得到许可的主机才能执行MA的代码，交换的数据也要求加密。当MA返回源主机后可根据MA执行过程中的信息判断是否有目标主机对其有恶意行为。分析了恶意主机上MA所受到的安全威胁，并用所提出的方案逐一分析解决了MA所受到的安全威胁。     

人肺腺癌细胞系A549细胞双向电泳-飞行时间质谱研究

目的初步分析人肺腺癌细胞系A549细胞蛋白质表达情况,从蛋白组水平探讨肺腺癌发病的分子机制。方法应用2-DE技术对人肺腺癌细胞系A549细胞总蛋白进行分离,获得蛋白质表达谱;应用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)结合生物信息学进行蛋白质鉴定。结果3块胶平均蛋白斑点数为1 138±49,平均匹配点数为986±32,匹配率为85.8%。随机选取背景清晰、分辨清楚、蛋白含量较高的20个斑点进行MALDI-TOF-MS分析,共获得了18个肽质量指纹图谱(PMF)。将PMFs质量数据通过Aldente软件查询SWISS-PROT数据库,根据匹配片段及氨基酸序列覆盖率等,初步鉴定出15种蛋白质。根据功能,这些蛋白质可分为:①基本代谢相关的酶类:果糖2-磷酸醛缩酶、视网醛脱氢酶1(RALDH1)、吡多醛激酶;②细胞骨架类:蛋白细胞角蛋白8(CK8)、-β2链微管蛋白;③应激相关蛋白:蛋白二硫化物异构酶A3(PDIA3);④信号转导分子:膜联蛋白1(ANX1)、膜联蛋白4(ANX4);⑤分子伴侣:热休克蛋白60(HSP60)、热休克蛋白-β1、抑制素(PHB);⑥转录及翻译相关蛋白:不均一性核糖核蛋白H(hnRNP H);⑦杂类:Rgr protein、NPM、PCBP1。结论应用2-DE/MALDI-MS方法获得了较为理想的人肺腺癌细胞系A549细胞2-DE蛋白质表达谱,初步鉴定了15种蛋白质。     

基于原子分解的辐射源信号二次特征提取

提出了基于原子分解的辐射源信号二次特征提取方法.在过完备多尺度Chirplet原子库基础上,首先用匹配追踪(MP)方法进行信号时频原子分解,并通过改进的量子遗传算法(IQGA)降低MP搜索过程的时间复杂性,得到表示雷达辐射源信号特征信息的最佳Chirplet原子.在此基础上,降低特征参数的维度,提取最具分类意义的原子特征向量.对5种典型雷达辐射源信号的特征提取实验表明,提取的原子特征类内聚集性强、类间分离度大,证实了本文方法的可行性和有效性.