绝经前妇女子宫切除前后心理状况调查分析

目的　了解子宫切除前后女性的心理状况改变趋势、程度和影响因素。方法　采用前瞻性研究方法调查 83例接受子宫切除手术女性入院时和术后 3月的心理状况。结果　心理状况调查自评得分显示术后BDI分值、SCL 90(偏执因子除外 )各因子得分显著低于术前 ,与他评调查HAMD评分一致 ,中、重度抑郁症发生率由术前 3 3 .7%降至术后 10 .1% ;不同年龄段患者SCL 90改变程度不同 ;术后BDI分值和HAMD得分与术前水平显著正相关 ;术前HAMD评分与受教育年限负相关 ,与主诉正相关 ,术后与产次、有内科合并症正相关 ,与年龄负相关。结论　子宫切除有利于患者身心健康 ;围手术期抑郁好发于年轻、多产、文化程度低、诉述痛经和月经过多及有其他内科疾患的妇女。     

c-FLIP蛋白与卵巢癌细胞对TRAIL耐药抵抗的关系

目的探索卵巢癌细胞对TRAIL诱导凋亡耐药抵抗的机制。方法分别选择12.5、25、50、100ng/mL的人重组TRAIL蛋白作用于卵巢癌细胞3AO和CAOV3,在18、24、48、72h时间点收集细胞,MTT法检测细胞的生长抑制率;原位末端标记法(TUNEL)观察凋亡细胞;流式细胞仪(FCM)检测细胞的凋亡率;Western blot检测凋亡抑制蛋白c-FLIP蛋白表达的水平。结果 TRAIL抑制了3AO和CAOV3细胞的生长增殖,24h3AO、CAOV3细胞的生长抑制率分别为28%、10%,并且TRAIL增多可诱导细胞凋亡,24h的3AO细胞凋亡率(8.5%)明显高于CAOV3(5.5%)。CAOV3细胞中c-FLIP蛋白的表达水平高于3AO细胞。结论 c-FLIP蛋白是卵巢癌细胞对TRAIL耐药抵抗的一种重要调控蛋白。     

PRELIMINARY STUDY OF A NOVEL HUMAN PAPILLOMAVIRUS TYPE 16L1／E6-E7 CHIMERIC RECOMBINANT DNA VACCINE

Objective Preparations of HPV16 L1/E6 and L1/E7 prophylactic and therapeutic DNA vaccines. Methods The nucleotides within HPV16 E6 and E7 genes, which are responsible for viral transforming activity, were mutated by mage primer site-directed mutagenesis method. The correctly mutated E6 and E7 fragments were separately cloned into an eukaryotic expression vector pVAX1, together with HPV16 L1 gene, generating chimeric recombinants plasmids 1MpVAX1-L1E6, 2MpVAX1-L1E6, 1MpVAX1-L1E7, 2MpVAX1-L1E7 and 3MpVAX1-L1E7. CHO cells were transiently transfected with the individual DNA vaccines by calcium phosphate method. Target protein expressions in the extracts of the transfected cell lines were measured by ELISA and immunohistochemistry, with HPV16 L1 and E6 specific monoclonal antibodies. Results ELISA assays showed the P/N ratios in the cell extracts transfected with L1E6 and L1E7 plasmids were more than 2.1. Immunohistochemistry revealed brownish precipitant signal in cytoplasm and nuclei of the transfected cells. Conclusion Successful constructions of prophylactic and therapeutic DNA vaccine plasmids lay solid foundation for future animal experiment and clinical trial.     

EXPERIMENTAL STUDY ON FREE VASCULARIZED FIBULA GRAFTING IN REVASCULARIZING FEMORAL HEAD

ＥＸＰＥＲＩＭＥＮＴＡＬＳＴＵＤＹＯＮＦＲＥＥＶＡＳＣＵＬＡＲＩＺＥＤＦＩＢＵＬＡＧＲＡＦＴＩＮＧＩＮＲＥＶＡＳＣＵＬＡＲＩＺＩＮＧＦＥＭＯＲＡＬＨＥＡＤＷａｎｇＫｕｎｚｈｅｎｇ；，ＷａｎｇＣｈｕｎｓｈｅｎｇ；，ＹａｎｇＷａｎｓｈｉ；ＬｉＸｕｄｏｎｇ...     

HUMAN PAPILLOMAVIRUS TYPE 16 L1 PROTEIN CAN BE EXPRESSED IN LIVE ATTENUATED SHIGELLA FLEXNERI 5A STRAIN SH42

Cervicalcanceristhesecondmostcommon canceramongwomenworldwide,anditaccountfor thehighestmortalityindevelopingcountries.There areabout600000newcasesdiagnosedanditcauses aboutaquartermillionwomendeathperyear[1]. Highrisktypehumanpapillomavirusinfection (suc…     

沙眼衣原体D型MOMP基因的克隆和序列分析

目的　从D型沙眼衣原体培养物中克隆主要外膜蛋白(MOMP)基因。方法　利用细胞培养法扩增沙眼衣原体后,提取基因组为模板,经聚合酶链反应(PCR)扩增全长MOMP基因,并用酶切、PCR扩增及序列测定等方法对重组质粒进行鉴定。结果　成功扩增了MOMP基因,并插入克隆载体 pUCmT中。结论　MOMP基因的克隆为进一步开展沙眼衣原体的疫苗研究奠定了基础。