THE STUDY ON THE CHANGES OF ZINC, COPPER, CALCIUM AND MAGNESIUM IN PLASMA AND ERYTHROCYTES DURING CARDIOPULMONARY BYPASS

Objective To study the changes and their influence factors involved of zinc, copper, calcium and magnesium in plasma and erythrocytes during cardiopulmonary hypass(CPB). Methods Zinc, copper, calcium and magnesium values in plasma and erythrocytes were measured by atomic absorption spectrophotometer during CPB. Results Zinc and copper levels in plasma were significantly elevated above preinduction level before perfusion, hut calcium and magnesium levels did not change significantly; zinc, copper and calcium levels in plasma were significantly below preoperation level during CPB, hut magnesium level in plasma was significantly increased above preoperation; zinc level in plasma was increased to preoperation level after CPB and began to decrease again at 8 hours after CPB,copper level in plasma was increased to preoperation level at 20 hours after CPB, calcium in plasma was increased significantly from beginning to 8 hours after CPB, magnesium level in plasma was decreased to preoperation level at 8 hours afterCPB. Concentration of zinc , copper, calcium and magnesium in erythrocytes did not change significantly. Conclusion During CPB, the changes of zinc, copper, calcium and magnesium had relation to hemodilution, operative wound, carrier protein, stress and component of priming Solution and cardioplegic solution, hut no relation to transferfrom plasma erythrocytes. The results indicate that it is beneficial to patient‘s recovery to supplement zinc, copper, calcium and magnesium properly by different ways during cardiac perioperation.     

AN EXPERIMENTAL STUDY ON REMOVAL OF NOx IN FLUE GAS AT THENONEQUILIBRIUM PLASMA

Removal of nitrogen oxides (NOx) in flue gas by means of nonequilibrium plasma technology is a very prospect and attractive method. As the nonequilibrium plasma micro discharges can generate a powerful energy flux, imparted to the flue gas, the molecules and atoms of pollutants are motivated and decomposed, and then NOx in the flue gas are decomposed and conversed in the particular conditions. Based on nonequilibrium plasma in combination with catalytic principle, an experimental investigation on NOx decomposition and conversion with AI2 03 catalysts was carriedout and the NOx removal rate up to 95% was obtained. The NOx decomposition and conversion principle with AI2O3 catalysts was also discussed.     

Determination and pharmacokinetic study of catechin in rat plasma by HPLC

A high performance liquid chromatographic method was developed and validated for the quantitative determination of catechin in rat plasma and its pharmacokinetic study after intragastric administration...     

Bio-analytical method development and validation of Rasagiline by high performance liquid chromatography tandem mass spectrometry detection and its application to pharmacokinetic study

The most suitable bio-analytical method based on liquid liquid extraction has been developed and validated for quantification of Rasagiline in human plasma. Rasagiline-13C3 mesylate was used as an inte...     

Development and validation for estimation of eplerenone of RP-HPLC method in spiked human plasma

A rapid and simple high performance liquid chromatography (HPLC) mcthod wiih a UV detection (241 nm) was developed and validated for estimation of eplerenone from spiked human plasma. The analyte and t...     

Spatter rate estimation of GMAW-S based on partial least square regression

This paper analyzes the drop transfer process in gas metal arc welding in short-circuit transfer mode （GMAW-S） in order to develop an optimized spatter rate model that can be used on line. According to thermodynamic characters and practical behavior, a complete arcing process is divided into three sub-processes： arc re-ignition, energy output and shorting preparation. Shorting process is then divided as drop spread, bridge sustention and bridge destabilization. Nine process variables and their distribution are analyzed based on welding experiments with high-speed photos and synchronous current and voltage signals. Method of variation coefficient is used to reflect process consistency and to design characteristic parameters. Partial least square regression （PLSR） is utilized to set up spatter rate model because of severe correlativity among the above characteristic parameters. PLSR is a new multivariate statistical analysis method, in which regression modeling, data simplification and relativity analysis are included in a single algorithm. Experiment results show that the regression equation based on PLSR is effective for on-line predicting spatter rate of its corresponding welding condition.     

Correlation between endothelia cells activation and imbalance of cytokines in pulmonary hypertension of congenital heart disease

Objective To explore the correlation between endothelia cells activation and cytokines （ET-1, NO） levels in patients with pulmonary hypertension （PH）, and to discuss their roles in the development of PH. Methods Twenty patients with simple ventricular septal defect （VSD） were chosen as controls, and 30 patients with PH were studied. Plasma levels of ET-1 and NO were measured by radioimmunoassay or colorimetric method. Before cardiopulmonary bypass was established, the specimens from right lung were fixed with formaldehyde solution, embedded with paraffin and stained by SP immunohlstochemistry. Intercellular adhesion molecule-1 （ICAM-1） expression was measured through the determination of the light density with computer imaging technology. Results Compared with that of the patients with simple VSD, the light density of ICAM-1 and plasma level of ET-1 increased in patients with PH; but plasma level of NO decreased （P〈0.05）. Positive correlation was observed between ICAM-1 and ET-1/NO （P〈0.05）. Conclusion Endothelia cells activation and imbalance of ET-1/NO might play an important role in the development of PH.     

EFFECT OF METHIONINE ENKEPHALIN ON MIGRATION OF MACROPHAGES FROM MICE WITH IMPAIRED LIVER

The increased plasma methionine enkephalin(M- Enk) concentration in patients with liver dis-eases indicates an alteration of the immune func-tion in these patients[1] .Since the effects of opioidpeptide on modulation of immune system in humanbeing and animals under normal condition havebeen revealed,more interest has recently been fo-cused on conditions in stress as liver impairment[2 ] .An attractive trend has emerged in the pastdecadethat in stress some neuropeptides may be secretedand relea…     

Inositol (1,4,5)-triphosphate Modulates Pollen Tube Polar Growth

Pollen tube is characterized by polarized elon-gation at the apex. Through polar growth, pollentube drives the sperm cells into the ovary to fulfillfertilization. So this process is essential for higherplants sexual propagation and food production[1].During finding its way to the ovary, pollen tubeshould always adjust its growth direction accordingto the external signals that are subsequently con-verted to the internal signals on plasma membrane.Ins(1,4,5)P3is one of such second messengers in…     

THE EUKARYOTIC EXPRESSION OF HUMAN PERFORIN PROTEIN AND THE ESTABLISHMENT OF HYBRIDOMAS PRODUCING ANTI-HUMAN PERFORIN PROTEIN

Perforin is expressed mainly in activated cyto-toxic T lymphocytes (CTLs ) and natural killer(NK) cells. The human perforin gene has beencloned. The full length of its cDNA is l668bp, andthe mature HP protein is made up of 534 aminoacid residues with a molecular weight of 66Kd~75Kd. In CTLs and NK cells, perforin is stored incytoplasmic granules and is a major effector of cy-tolysis by these cells. Upon granules releasing,perforin monomers insert into the plasma mem-branes of target ce…     

城市轨道交通靴轨关系的安全测试评估方法

为实现城市轨道交通初期运营前靴轨关系的安全评估,采用激光摄像、紫外光电传感、光纤光栅应变传感技术对接触轨动态几何参数、靴轨燃弧、靴轨动态接触力、集电靴硬点进行检测. 根据各项检测参数的特征分别确定各参数评定标准;选择接触轨工作高度、接触轨偏移值、靴轨燃弧率和靴轨动态接触力4项检测参数,采用Topsis评价方法构建接触轨质量指数评价函数;基于各参数评定标准和质量指数评价函数提出一套城市轨道交通初期运营前靴轨关系安全评估方法;利用运营车靴轨关系检测装置对广州地铁21号线镇龙西—员村段进行了全线数据检测. 研究结果表明:全线接触轨动态几何参数与设计值偏离均不超过 ± 5 mm,最大燃弧时间为53 ms,最大燃弧率为0.025%,区间平均接触力最大值和最小值分别为135.6 、119.8 N,集电靴硬点最大为29.8 g,全线质量指数评分 大于 92.89分.      

基于弓网电弧模拟试验装置的电弧参数测量及分析

电气化高速铁路弓网相互作用是实现机车受流的关键环节,弓网电弧是评价弓网关系的重要特征.本文设计了一套弓网电弧模拟发生装置,该装置通过旋转轮盘与碳刷相对运动,产生旋转电弧,能够模拟受电弓与接触网的相对运动.系统试验最高电压100 V,电弧电流20 A,试验测试了静态电弧和动态电弧对系统的电压和电流的影响,并采用LABVIEW软件对其进行了谐波分析.谐波成分集中在500 Hz以下,且含有不规则间谐波.     

高速铁路自然过分相方案

在深入研究机车带负荷过分相产生电弧机理的基础上,提出了自然过分相技术方案.该方案的基本原理是在车载变压器高压侧和牵引供电臂末端分别并联一个电容,当机车过分相时,牵引网和车载电气传动系统实现等电位分离,避免了机车过分相时产生电弧.自然过分相方案和现在使用的3种方案相比,具有过分相断电时间短,结构简单,投资少,运行维护量小等优点.自然过分相方案还能大幅度降低机车运行时离线电弧的强度.     

招弧角电极直流电弧运动特性分析

接地极线路是直流输电系统的重要组成部分，是直流系统不平衡电流和大地回流的入地通道.接地极线路遭遇雷击时，入地电流会在招弧角间隙形成稳定电弧，由于直流电流不存在周期性过零点，直流电弧很难熄灭，进而破坏线路绝缘，严重威胁直流输电系统安全.针对接地极线路招弧角处于野外开放空间、电弧运动受多场耦合影响的特点，建立了招弧角电弧磁流体动力学二维仿真模型，研究气流速度、方向以及招弧角结构对直流电弧运动特性的影响.研究表明：招弧角电极电弧主要受电磁力作用沿电极扩张方向运动，通过吹弧和拉伸，降低电弧温度，提高电弧电压，使电弧更利于熄灭；气流环境对电弧动态特性产生较大影响，同方向风速有利于电弧的吹离和疏导；同等风速下，水平方向气流吹弧效果更显著；电极样式对电弧的拉伸有重要影响，同等间隙距离下，双羊角电极对电弧的拉伸作用比单羊角电极更强；在入地电流持续注入的情况下，电弧易重燃，且难以彻底熄灭.     

Research on arc erosion of silver-based alloy contact materials under low voltage, resistive load and small current at 400 Hz and 50 Hz

By using a self-developed IF power and a ASTM contact material experimental system of small-capacity and variable frequency, the value of arcing characteristics and the welding force of the silver-based contact material are acquired under low voltage, resistive load and small current at 400 Hz and 50 Hz. By means of an electricity-ray analytical balance, SEM and EDAX, the weighing values of the contact materials and the changes of AgCdO, AgNi, AgC and AgW contact material surface profile and micro-area constituent are obtained and analyzed. The arc erosion causes of silver-based alloy contact materials at 400 Hz and 50 Hz are also discussed.     

弓网离线模拟试验平台的设计

受流问题是制约电气化铁路提速的瓶颈之一,而弓网离线电弧是高速列车受流的关键问题.为了全面的把握弓网离线状态,设计了弓网离线模拟试验平台.实现正弦周期内不同时刻离线及回合过程的电弧放电现象,并采用示波器和数据采集卡实现对离合瞬间电压、电流和光强等波形的同步记录,为弓网离线检测法和列车受流质量的研究及抑制电弧危害打下基础.     

压力传感器灵敏度特性的研究

针对应变片在圆平薄膜上的两种布置方式，导出了圆平薄膜压力传感器灵敏度的计算公式，分析了应变片布置方式和位置以及温度对灵敏度的影响，通过理论计算与实际测量的结果比较，指出了提高压力传感器灵敏度的有效途径，并且分析了应变片两种布置方式压力传感器灵敏度与温度的关系。     

驻点热流测量试验技术研究

为了提高超声速脉冲风洞中球头驻点热流测量精度,对1 mm小型化同轴热电偶研制及薄膜热流传感器的耐冲刷性能进行研究.应用热传导反问题的计算分析方法,建立了分别针对薄膜热流传感器和同轴热电偶的数据处理方法.在2 m激波风洞上利用研制的5种不同类型及尺寸的传感器进行了球头驻点热流测量试验,将试验结果与F-R公式计算结果进行比较,并分析了传感器外形尺寸、热壁、传感器敏感元件、同轴热电偶膜厚等对试验结果的影响.结果表明:研制的小型化同轴热电偶响应时间满足测试要求;薄铂膜热流传感器重复性误差在8%以内.      

阵列式等离子体射流特性的研究

研究了大气压下阵列式等离子体射流特性,以实现和完善大气压下大面积的低温等离子体灭菌技术.采用MAXWELL 3D软件仿真及实验的手段,研究相关因素对各种阵列式等离子体射流特性的影响.包括在单管等离子体喷射中的一些基本影响因素（电压、气体流速等）和阵列式等离子体喷射实验中所特有的一些因素（气流均匀度、电极单元间距及电极布置方式）.实验在大气压氦气环境下进行,然后通过专门研发的高频高压等离子体放电电源进行了介质阻挡放电.实验结果实现了大气压下阵列式等离子体喷射,证明了通过阵列式等离子体喷射方式来实现大面积等离子体灭菌技术的可行性.除此之外还得出了各种相关因素对阵列式等离子体射流特性的具体影响,用以完善阵列式等离子体射流技术,进行更大面积的灭菌处理.