集装箱船线型优化研究

为了充实船型数据库及满足将来可能的市场需求,结合传统经验设计方法和CFD技术同步进行了集装箱船的线型研发。通过两个方案的船模试验结果的比较,得出一些有意义的结论,对将来的线型设计工作具有一定的启迪意义。     

海洋作业工程船空调设计

随着海上石油勘采、矿产资源开发的日益升温,海洋作业工程船是近几年市场需求量较大的一类船型,由于船员配置及作业环境的特殊性,空调系统的设置同常规船舶相比有其特殊的要求。同时随着造船技术的发展,人们对船舶空调系统的舒适性、经济性的要求也越来越高。该文通过4 000 t全回转起重打捞工程船空调系统设计,对此类工程作业船舶空调设计方面的一些问题进行论述,可供读者参考。     

VW-MSPC方法在变压器状态评估中的应用研究

针对变压器状态评估变量指标之间具有相关性以及重要性不同的特点,在基于马氏距离的传统多元统计控制图中引入加权马氏距离,设计一种基于变量加权的多元统计过程控制的变压器评估方法。该方法结合关联规则方法给出的变压器关键指标变量体系利用多元控制图的波动检出能力,将变压器各类故障对应的多元实时监测数据转化为一个检验统计量,进而对变压器运行状态进行评估。电流回路过热故障的实例分析表明,相对于现行的变压器状态评估标准,该方法具有更快的故障检出速度;对于已知故障的样本数据的回顾检测表明该方法具有较高的故障检出率。     

Collision avoidance path planning in multi-ship encounter situations

Journal of Marine Science and Technology - Collision avoidance path planning is still one of the essential problems in the design and application of an intelligent maritime navigation system. Its...     

新疆四县农村3岁以下儿童营养状况分析

目的　了解新疆维吾尔族自治区 (简称新疆 )农村 3岁以下儿童的营养状况 ,为针对性开展干预提供决策依据。方法　对新疆 4县进行了随机抽样横断面调查 ,以身高和体重作为衡量儿童营养状况的指标。结果　该地区 3岁以下儿童生长迟缓 (HAZ <- 2 )、低体重 (WAZ <- 2 )和消瘦 (WHZ <- 2 )的总患病率分别为 2 4.3 %、2 3 .1%和 9.1% ;维吾尔族高于汉族 ;经济差的县高于经济好的县。与NCHS/WHO标准分布比较 ,新疆儿童HAZ和WAZ的分布显著左移。结论　新疆农村 3岁以下儿童存在着较严重的营养问题。应积极开展营养健康教育 ,加强儿童常见病的防治。在重点干预的同时 ,还要注意改善整体儿童的营养状况     

TRAIL在食道鳞癌及癌旁组织中的表达

目的　研究人肿瘤坏死因子相关凋亡诱导配体 (TNF relatedapoptosisinducingligand ,TRAIL)在食道鳞癌及癌旁组织中的表达及意义。方法　采用免疫组化S -P法 ,检测 42例食道鳞状细胞癌及其癌旁组织中TRAIL蛋白表达水平。结果　TRAIL在正常食道粘膜上皮、单纯增生、不典型增生、鳞癌组织中其阳性表达呈递减趋势 (P <0 .0 1) ;在Ⅰ、Ⅱ级鳞癌明显高于Ⅲ级和未分化鳞癌 (P <0 .0 1)。而在早期鳞癌和晚期鳞癌中的表达无差异 (P >0 .0 5 )。伴有淋巴结转移组和无转移组之间无差异 (P >0 .0 5 )。结论　TRAIL的表达可能与食道鳞癌的发生、发展密切相关 ;与食道鳞癌的分级呈负相关 ;与癌组织浸润深度和淋巴结转移无关。     

GUSTATORY RESPONSES OF NEURONS IN THE PARABRACHIAL NUCLEUS OF RATS

Ａｎｕｍｂｅｒｏｆａｎａｔｏｍｉｃａｌ,ｅｌｅｃｔｒｏｐｈｙｓｉｏｌｏｇｉｃａｌａｎｄｂｅｈａｖｉｏｒａｌｅｘｐｅｒｉｍｅｎｔｓｈａｖｅｓｈｏｗｎｔｈａｔｇｕｓｔａｔｏｒｙｉｎｆｏｒｍａｔｉｏｎｉｎｔｈｅｎｕｃｌｅｕｓｏｆｔｈｅｓｏｌｉｔａｒｙｔｒａｃｔ(ＮＴＳ) ,ｗｈｉｃｈｉｓｃｏｎｖｅｙｅｄｆｒｏｍｔｏｎｇｕｅｖｉａｔｈｅｃｒａｎｉａｌｎｅｒｖｅｓ (ｆａｃｉａｌ,ｇｌｏｓｓｏｐｈａｒｙｎｇｅａｌ,ａｎｄｖａｇｕｓ) ,ｒｅａｃｈｅｓａｃｏｎｆｉｎｅｄ ｐａｒｔｏｆｔｈｅ ｐａｒａｂｒａｃｈｉａｌｎｕｃ…     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.     

单纯疱疹病毒2型gG-2"独特区"基因的原核表达及其血清学诊断价值

目的在大肠杆菌中表达HSV-2的gG-2"独特区"基因(gG-2T)并对其免疫学特性进行初步研究。方法克隆HSV-2的gG-2T,并在大肠杆菌中表达gG-2T融合蛋白。通过Western blot和间接ELISA鉴定融合蛋白的活性。结果通过原核系统表达的gG-2T融合蛋白可以与GST多克隆抗体结合并在46 ku附近出现特异性结合条带。并且能够与确诊的HSV-2患者血清反应,而不与HSV-1患者血清和正常人血清反应。结论获得了HSV-2 gG-2T融合蛋白,它可以与GST多克隆抗体特异性结合并且可以同HSV-2型患者血清特异性反应;获得的gG-2T蛋白为研究以gG-2蛋白作为靶区域的诊断试剂盒和疫苗研发打下实验基础。     

PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.