Effects of Vitamin E and Selenium on Free Radicals Metabolism of Broilers

Since Haman (1982) firstly postulated freeradical theory, the metabolisms and functions offree radicals in animal organisms has arouse greatconcerns in the biology research field. In the pro-cedure of normal organism material metabolism,there exists dynamic balance among the producing,utilizing and clearing of free radical. VE is an im-portant antioxidant in the organism and breaksperoxidation of lipids associated with vitamin C(VC)[1,2]. Selenium (Se), as part of the active siteof GSH-Px[…     

Effect of ABCA1-V771M polymorphism on plasma lipid levels and its relationship with coronary atherosclerotic heart disease

Objective To explore the risk association of ABCA1-V771M polymorphism with coronary heart disease (CHD) in Hart nationality in Northwest of China. Methods With case-control study, ABCA1-V771M polymorphism was detected in 204 unrelated Hart nationality people in Northwest of China, and all the subjects by coronary angiography were grouped into 106 cases and 98 controls. The genotypes and alleles frequency distribution of ABCA1-V771M polymorphisms were analyzed by PCR-RFLP analysis, and the clinical statistics of serum lipids were compared and its effects of ABCA1-V771M polymorphism on the plasma lipid levels and coronary atherosclerotic heart disease were analyzed. Results The genotypic frequencies of ABCA1-V771M polymorphism matched well under Hardy-Weinberg equilibrium (P>0.05), V and M allelic frequencies were 33.3% and 66.7%. In comparison with VV VM genotype carriers, MM genotypes carriers had much lower plasma levels of HDL-C (P<0. 001) and much higher plasma levels of TG (P<0. 05). M allelic frequency in CHD group was significantly higher than V allelic frequency (P<0. 05). M allele was related with more severity of atherosclerosis in the coronary artery than V allele (P<0.05). However, there was no obvious difference in the incidence of AMI among carriers with three genotypes of ABCA1-V771M polymorphism (P>0.05). Conclusion ABCA1-V771M polymorphism was not only associated with the plasma levels of HDL-C and TG, but also related to the susceptibility and severity of coronary atheroselerotic heart disease. Moreover, M771 allele appeared to be atherogenie among Han population in Northwest of China.     

血清胱抑素C与脑梗死发病相关性的临床研究

目的研究血清胱抑素C(Cys-C)与脑梗死的相关性。方法回顾性分析比较180例脑梗死患者与112例对照组患者的血清Cys-C水平及相关临床资料。结果①脑梗死组患者血清Cys-C水平(0.95±0.23 mg/L)较对照组(0.76±0.14 mg/L)明显增高(P<0.01);②血清Cys-C水平与患者性别有关,且与年龄(r=0.180,P=0.016)、同型半胱氨酸(r=0.257,P=0.001)呈正相关,与高密度脂蛋白(r=-0.219,P=0.003)呈负相关;③Logistic回归分析显示,年龄(OR=1.063,P<0.001)、吸烟(OR=8.178,P<0.001)、高血压(OR=7.649,P<0.001)、糖尿病(OR=4.798,P=0.011)及血清Cys-C(OR=1.675,P=0.007)均是脑梗死的危险因素。经控制其他危险因素后,血清Cys-C仍与脑梗死密切相关(r=0.215,P<0.01)。结论血清Cys-C水平的升高是脑梗死的独立危险因素。     

单纯多滤泡卵巢妇女的糖代谢水平的变化

目的研究单纯多滤泡卵巢(MOF)妇女的糖代谢水平,探讨其与胰岛素抵抗及体重的关系。方法46例MOF妇女及30例正常对照女性,口服75g葡萄糖,行糖耐量试验(OGTT)及同步胰岛素释放试验(IRT),用稳态模型胰岛素抵抗指数(HOMA IR)测评胰岛素抵抗。结果①MOF糖耐量低减的发生率为10.87%,明显高于对照组3.33%(P<0.05)。胰岛素抵抗的发生率为30.43%,对照组为10.00%,两组比较差异有统计学意义(P<0.05)。②两组的性激素水平,即FSH、LH、PRL、E2、T和P的差异无统计学意义(P>0.05)。③糖耐量低减妇女BMI与胰岛素抵抗呈正相关(r=0.567,P<0.05)。结论单纯多滤泡卵巢妇女存在一定程度的糖代谢异常;肥胖和胰岛素抵抗是导致糖耐量异常的主要原因。MOF肥胖妇女应行OGTT监测糖代谢状况。     

维甲酸致颌骨骨质疏松大鼠骨代谢变化及“双骨胶囊”治疗效果

目的　观察维甲酸致颌骨骨质疏松大鼠骨代谢变化和中药“双骨胶囊”的治疗效果。方法　将 6 0只 3月龄SD雌性大鼠随机分为二组 :正常组、模型组。模型组大鼠是以 85mg·kg-1·d-1维甲酸灌胃 15d复制模型 ,模型复制成功后进入治疗期。将模型组大鼠随机分为三组 :无措施组、双骨胶囊组和雌激素组。治疗 30d、6 0d后 ,取大鼠血、尿、下颌骨样本 ,观察治疗情况。结果　“双骨胶囊”治疗后 ,维甲酸所致颌骨骨质疏松大鼠的下颌骨骨小梁增宽 ,骨小梁之间间隔减少 ,骨髓腔缩小。骨小梁平均宽度增加 ,骨小梁平均间隔宽度减少。血清雌二醇水平显著升高 ,破骨细胞分泌的抗酒石酸酸性磷酸酶活性降低 ,血清骨钙素含量升高 ,尿钙增加。结论　治疗骨质疏松的药物“双骨胶囊”不仅对全身骨起作用 ,对治疗颌骨骨丢失也有一定的效果     

丹红注射液对U937细胞SR-AⅠ及ABCA1 mRNA表达的影响

目的 以人单核细胞株U937细胞为研究对象,观察不同浓度丹红注射液对U937细胞清道夫受体AⅠ(SR-AⅠ)和ATP结合盒转运体A1( ABCA1)mRNA表达的影响,探讨其抗动脉粥样硬化的机制.方法 以100 nmol/L佛波酯诱导U937细胞48 h使其分化为巨噬细胞,再以50 mg/Lox-L DL(氧化低密度脂蛋白)孵育细胞的同时加入不同浓度丹红注射液或其他干预因素处理24 h,用实时荧光定量PCR方法检测其SR-A Ⅰ和ABCA1 mRNA的表达量.结果 与50 mg/L ox-LDL组比较,LXR-a(肝X受体激动剂)(T-090137)干预组U937细胞SR-A ⅠmRNA表达下降、ABCAl mRNA表达升高,但SR-A Ⅰ mRNA的表达无统计学意义(P＞0.05),而ABCAl mRNA的表达有统计学意义(P＜0.01);3.0 mL/L丹红注射液干预组U937细胞SR-A Ⅰ mRNA的表达升高,10.0、30.0、60.0 mL/L丹红注射液于预组U937细胞SR-A Ⅰ mRNA的表达逐渐下降,但各浓度组均无统计学意义(P＞0.05);丹红注射液干预组ABCAl mRNA的表达则呈浓度依赖性升高,但于60.0mL/L浓度时出现表达下降,3.0、10.0、30.0 mL/L干预组有统计学意义(P＜0.05).与LXR-a(肝X受体激动剂)(T-090137)干预组比较,丹红注射液各浓度干预组ABCAl mRNA表达降低,10.0、30.0 mL/L丹红注射液干预组ABCA1 mRNA的表达无统计学意义(P＞0.05).结论 丹红注射液各浓度组对50 mg/Lox-LDL干预的U937细胞SR-A Ⅰ mRNA的表达无影响,而明显增加U937细胞ABCA1 mRNA表达,尤其在浓度10.0、30.0 mL/L时增加ABCA1 mRNA表达明显.增加U937细胞ABCA1 mRNA表达可能是其抗动脉粥样硬化的重要机制.     

灰色系统理论在水泥稳定碎石施工质量控制中的应用

根据灰色系统理论的预测,针对水泥稳定碎石施工质量控制,以强度控制指标为例建立灰色模型,并采用灰色绝对关联度分析模型的精度,同时引入新陈代谢模型来增强预测模型的适应性.通过模型计算值与实际值的比较分析可知,该模型具有合理性和可靠性.     

In situ absorption and metabolism of stilbene glycoside in rat

Stilbene glycoside (TSG) has been shown to have many beneficial properties. It is therefore essential to understand the absorption and metabolism of TSG in detail. We determined the recovery of TSG and its metabolites (TSG sulfate/glucuronides) in rat gastric contents, gastric mucosa, portal vein plasma, celiac arterial plasma, bile, and urine after administration of 15mg of TSG in 0.5mL physiological saline or incubation for 20min in situ in the stomach of rats. Within 20min, (64.0±9.8)% of the administered TSG disappeared from the stomach; later, TSG was recovered in both free and conjugated forms in plasma and bile, but not in urine. On the other hand, only free TSG was detected in the gastric contents and mucosa; it was also detected in the portal vein plasma as (48.1±3.5)% of the total TSG (all forms of TSG). However, the proportion of free TSG in the celiac arterial plasma and bile decreased to 4%-10%. In addition, the proportion of free TSG to total TSG in the liver microsome incubation mixture after TSG was incubated in liver microsome at 37℃ for 30min was very low [(10.6 ± 2.6)%]. These results indicate that TSG could be quickly absorbed from the rat stomach, conjugated in liver and excreted in bile. Such novel information would be helpful for the use of TSG as a beneficial natural product which may improve its proposed efficacy in preventing chronic diseases.     

铅对儿童血液多种金属元素代谢的影响

动物实验和铅暴露儿童调查发现,铅可干扰体内钙、锌、铁、铜、镁等金属元素的代谢,进一步诱导铅毒性的产生。提示膳食或强化补充因铅暴露而引起缺乏的元素能抑制肠道对铅的吸收,降低铅毒性。     

尼古丁对巨噬细胞肝X受体α表达及胆固醇外流的影响

目的通过研究尼古丁对巨噬细胞的肝X受体α(LXRα)及其下游的基因表达和胆固醇外流的影响,探讨尼古丁对LXR信号系统的作用。方法分离人外周血单核细胞,并转化为巨噬细胞。在尼古丁的作用下,观察巨噬细胞的aopA-Ⅰ介导的胆固醇外流的变化和LXR及其下游一些基因mRNA表达。结果尼古丁显著影响巨噬细胞中一些涉及胆固醇代谢及炎症反应的基因表达,同时降低aopA-Ⅰ介导的胆固醇外流。结论尼古丁影响巨噬细胞LXR信号途径,促进泡沫细胞形成,使动脉粥样硬化发生发展。