孤独求败 2013帕加尼Zonda Revolucion

外观上,工程师为其设计了诸多优化空气动力学的动力套件,相比起常规Zonda,Revolucion在前引擎盖上加载了全新的反射器,在车身后部加载了垂直车身稳定器,大大提升了车身稳定性,减少了空气阻力,提升车身下压力,让它在直道和弯道都有了更优秀的表现。尾部我们还看到了类似F1赛车中的超大尾翼,并装有DRS(Drag-Reduction-System)减阻系统。该系统     

旧水泥混凝土路面破碎工艺应用与探讨

水泥路面采用水泥路面破碎机进行破碎的新工艺,缩短了工期,优化了结构,节约了投资。使资源得以就地再生利用,在减少了废料丢弃的同时、又保护了生态环境,实现了资源环境可持续发展,具有十分明显的环境效益、经济效益和社会效益。     

ETC技术助推我国智慧高速公路发展

我国高速公路全面实施的ETC收费技术取得了显著成就,提高了高速公路管理水平和标准化体系建设,完成了基础设施升级改造工程,加快了高速公路数字化、网络化和智能化进程,为向未来智慧高速公路平滑过渡奠定了坚实基础,同时,带动了5G技术商用及智能汽车产业加速发展,推进了智慧高速公路示范区建设,成为助推我国智慧高速公路发展的引擎。     

农村公路建设存在问题和应采取的措施

从2004年开始,河北省农村公路进入了大规模建设发展时期,大规模的农村公路建设极大地改善了农村交通条件。促进了农村经济发展．优化了投资环境,对加快农村产业结构调整。发展现代农业和增加农民群众收入．发挥了重要作用。同时,农村交通条件的改善．提高了农民生活质量,改善了村容村貌．推动了乡风文明和基层党组织建设．密切了党群、干群关系．     

基于同级库存协同的装备调配方案优化研究

文章讨论了动态需求下同级库存协同的重要性,分析了装备调配方案的制定流程,给出了同级库存调剂方案的前提.考虑供应成本和供应满意度的平衡,从运输成本、库存积压和装备短缺损失等方面,研究了上级调拨和同级调剂的成本构成.综合了上级调拨和同级调剂的优点,建立了一个装备调配的最优化模型,得到了最优调配方案.最后用一个实例验证了模型的可行性和有效性.     

简述凤凰岭隧道进口高边坡斜交进洞施工工法

忻州—阜平高速公路忻州至长城岭段凤凰岭隧道进口段（马家庄村）的进洞方案选择中,成功地解决了高边坡斜交进洞的难点,保证了安全,提前了工期,节约了成本,保护了生态。通过总结各施工难点,形成了本施工工法。     

浅谈全站仪在公路建设中的应用

全站仪与GPS的应用大大提高了测量的精度 ,降低了劳动强度 ,提高了准确性 ,节省了作业时间 ,介绍了全站仪在公路建设中的应用     

浅谈高速公路绿化的作用

随着人们环境保护意识的提高,公路交通运输业和国家可持续发展战略的逐步推进,对公路的环境建设和环境保护尤为重视。各条高速公路在建设期间都投入大量资金进行了绿化,有的甚至进行了综合景观设计,建成了景观大道,不但稳固了路基,保护了环境,美化了路容,还给司乘人员减轻了疲劳,降低了交通事故。     

试论路况管理系统的开发使用与作用

论述了阳泉郊区公路管理段利用网站平台,结合公路养管实际,开发了路况管理系统,并对该系统的开发、使用、具体内容、作用等进行了详细的阐述,反映了管理人员通过该系统可以便捷地查询、分析、对比各项数据,大大提高了工作效率,从而为日常养护管理、分析、决策提供了有力的信息保障。     

罩面类预防性养护方法

薄层罩面作为一项预防性养护技术,给原沥青路面提供一个崭新的表面,使原沥青路面的平整度大大增加,减小了行车的振动,减少了行车对路面的激振破坏并增加了行车的舒适性;恢复了表面粗糙度,使抗滑能力提高,增加了行车的安全性使路面原有的许多表面破坏,如坑洞、裂缝、辙槽等都得到了一定程度的治理,并延长了路面使用寿命。     

Microstructures and mechanical properties of various Mg-Li wrought alloys

Three kinds of ultra-light Mg-Li wrought alloys,Mg-5.5Li-3Al-1Zn-1Ce,Mg-7.0Li-3Al-1Zn-1Ce and Mg-10.5Li-3Al-1Zn-1Ce,were prepared by vacuum induction melting furnace;then the alloys were extruded and rolled.The microstructures and properties of these alloys were studied.The results indicate that the Mg-5.5Li3Al-1Zn-1Ce alloy has the highest strength,the Mg-10.5Li-3Al-1Zn-1Ce alloy has maximum elongation,and the Mg-7.0Li-3Al-1Zn-1Ce alloy has good comprehensive performance.Rolling deformation can increase strength further after extrusion deformation.     

Genetic polymorphism of T6235C mutation in 3' non-coding region of CYP1A1 and GSTM1 genes and lung cancer susceptibility in the Mongolian population

Objective To estimate the relative risk for lung cancer associated with genetic polymorphism of T6235C mutation in 3' non-coding region (Msp Ⅰ) of cytochrome P450 1A1 (CYP1A1) and glntathione S-transferase M1 (GSTM1) in the Mongolian population in Inner Mongolian Region of China. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiplex PCR methods were used to analyze blood samples obtained from 263 case subjects and 263 control subjects to determine their genotypes for CYP1A1 and GSTM1.Control subjects were matched with case subjects by ethnic background, age and gender. Results The frequencies of the variant CYP1A1 genotypes (CYP1A1C) and GSTM1-null in lung cancer groups were higher than those in control groups (38.4% vs. 28. 5% and 57.8% vs. 48.0%). The individuals who corried with CYP1A1C genotype had a significantly higher risk of lung cancer (OR=1.56, 95% CI=1.08 to 2.25, P=0.016) than those who carried with non-variation CYP1A1 genotype. The ones who carried with GSTM1-null genotype also had a significantly higher risk of lung cancer (OR=1.49, 95% CI=1.06 to 2.10, P=0.023) than these who carried with GSTM1-present genotype.When combination of polymorphisms of CYP1A1 and GSTM1 genotypes was analyzed, the risk of lung cancer for combination of CYP1A1C and GSTM1-null genotypes was increased significantly (OR=2.084, 95e CI=1.27 to 3.42, P=0.003). Susceptibility to lung cancer was related to smoking (OR=2.10, 95% CI=1.48 to 2.98, P=0.000). Considering smoking status, the risk of lung cancer for combination of smoking and CYP1A1C genotype was remarkably increased (OR=2.76, 950/0 CI=1.74 to 4. 37, P=0.000). It was the same case with combination of smoking and GSTM1-null genotype (OR=4. 38, 95% CI=2.35 to 8.15, P=0.000). Conclusion The polymorphisms of CYP1A1C genotype and GSTM1-null are the risk factors of lung cancer in the Mongolian population in Inner Mongolia Region of China. Smoking is also related to susceptibility to lung cancer. There may be a synergetic interaction between CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer. Smoking may have a synergetic interaction with CYP1A1C and GSTM1-null in the elevated susceptibility of lung cancer.     

IDENTIFICATION OF HUMAN MURR1, THE HOMOLOGUE OF MOUSE IMPRINTED Murr1 GENE

Different fromthe biallelic expression mannerof most common autosomal genes,i mprinting is aspecial genomic expression phenomenon,of whichthe i mprinted genes show parental-specific expres-sion only according to their parental origin[1].Cor-rect i mprintingis i mportant inthe nor mal embryon-ic development,postnatal growth,as well as behav-ior of ani mals.Dysregulation of i mprinting hasbeen i mplicated in the pathogenesis of congenitaldiseases and cancer[2-3].To date,78kinds of mousei mprinte…     

Construction and identification of recombination expression vector Ksp-Cadherin-Gpx1-Kik1

Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin eDNA were obtained by taking Gpx1 cDNA, Klk1 eDNA, and Ksp-cadherin BAC as templates. After being testified, the PCR products were inserted into the expressive vector pIRES-EGFP step-by-step to produce a recombinant vector Ksp-cadherin-Gpx1-Klk1. This vector was examined by restriction enzyme digestion and sequence analysis. Results The recombinant expressive vector Ksp-cadherin-Gpx1-Klk1 was successfully constructed. Conclusion The construction of the recombinant vector Ksp-cadherin-Gpx1-Kik1 laid foundations for investigations in establishing transgenic animal models, the over-expression of Gpx1 and Kikl in mammal kidney, and gene therapy for ischemia-reperfnsion injury during kidney transplantation.     

PRELIMINARY STUDY OF A NOVEL HUMAN PAPILLOMAVIRUS TYPE 16L1／E6-E7 CHIMERIC RECOMBINANT DNA VACCINE

Objective Preparations of HPV16 L1/E6 and L1/E7 prophylactic and therapeutic DNA vaccines. Methods The nucleotides within HPV16 E6 and E7 genes, which are responsible for viral transforming activity, were mutated by mage primer site-directed mutagenesis method. The correctly mutated E6 and E7 fragments were separately cloned into an eukaryotic expression vector pVAX1, together with HPV16 L1 gene, generating chimeric recombinants plasmids 1MpVAX1-L1E6, 2MpVAX1-L1E6, 1MpVAX1-L1E7, 2MpVAX1-L1E7 and 3MpVAX1-L1E7. CHO cells were transiently transfected with the individual DNA vaccines by calcium phosphate method. Target protein expressions in the extracts of the transfected cell lines were measured by ELISA and immunohistochemistry, with HPV16 L1 and E6 specific monoclonal antibodies. Results ELISA assays showed the P/N ratios in the cell extracts transfected with L1E6 and L1E7 plasmids were more than 2.1. Immunohistochemistry revealed brownish precipitant signal in cytoplasm and nuclei of the transfected cells. Conclusion Successful constructions of prophylactic and therapeutic DNA vaccine plasmids lay solid foundation for future animal experiment and clinical trial.     

CYP2E1-RsaⅠ、GSTT1基因多态性与胰腺癌遗传易感性的病例对照研究

目的 探讨吸烟和细胞色素P4502El-RsaⅠ(CYP2E1-RsaⅠ)、谷胱甘肽硫转移酶T1(GSTT1)基因多态性与胰腺癌发病之间的关系.方法 采用病例-对照研究的方法,以150例胰腺癌患者及150例非癌对照者的外周血白细胞为样本,利用聚合酶链反应(polymerase chain reaction,PCR)技术分析了Ⅰ相代谢酶CYP2E1-RsaⅠ和Ⅱ相代谢酶GSTT1基因多态性.结果 CYP2E1-RsaⅠ野生纯合型(c1/c1)和GSTT1基因缺陷型频率分布分别为38.7%、69.3%(病例组)和20.7%、44.7%(对照组),二者经χ2检验差异有显著性(χ~2=15.75,P<0.01;χ~2=18.62,P<0.01).c1/c1基因型者患胰腺癌的风险显著增加(OR=3.19,95% CI=2.53～4.26).GSTT1(-)者患胰腺癌的风险也显著增加(OR=2.85,95% CI=1.92～4.64).基因突变的协同分析发现CYP2E1-RsaⅠ(c1/c1)/GSTT1(-)在胰腺癌组和对照组中的分布频率分别为30.7%和6.7%,二者经χ2检验有显著性差异(χ~2=42.39,P<0.01).CYP2E1-RsaⅠ(c1/c1)/GSTT1(-)患胰腺癌的风险显著增加(OR=16.63,95% CI= 8.94～22.01).病例组的吸烟率显著高于对照组的吸烟率(OR=2.74,95% CI=1.32～4.58,P<0.01),CYP 2E1-RsaⅠ(c1/c1)及GSTT1(-)与吸烟有协同作用(OR=8.84,95% CI=5.51～11.62;OR=20.40,95% CI=4.98～29.53).结论 CYP2E1-RsaⅠ(c1/c1)和GSTT1(-)是胰腺癌的易患因素,二者对胰腺的发生有协同作用,吸烟与胰腺的易感性也有关,CYP2E1-RsaⅠ(c1/c1)、GSTT1(-)与吸烟在胰腺癌的发生上也有相互促进作用.     

拟常曲率空间中具常平均曲率的闭超曲面

设(Nn+1,g)是n+1维单连通完备黎曼流形,其黎曼曲率张量取如下形式:KABCD=a(gACgBD-gADgBC)+b(gACλBλD-gADλBλC+gBDλAλC-gBCλAλD),则称Nn+1为拟常曲率空间。又设M是Nn+1中具常平均曲率的连通闭超曲面,S为M的第二基本形式模长的平方。若Nn+1的生成元切于M,则(1)当S2(n-1)~(1/2)(a+b-b)时,M是全脐超曲面;(2)当S=2(n-1)~(1/2)(a+b-b)时,M是全脐超曲面或球面Sn+1(a)中的H(r)-环面S1(r)×Sn-1(t)。若Nn+1的生成元法于M,则(1)当S=2(n-1)~(1/2)a时,M是全脐超曲面;(2)当S=2(n-1)~(1/2)a时,M是全脐超曲面或Nn+1中的H(r)-环面S1(r)×Sn-1(t)。     

关于欧几里得子空间正交补的正交基

关于欧式空间子空间正交补的正交基的计算,本文提出比schmidt process简单的一种算法。在n维欧式空间中,我们取一线性无关组M={α1,…,αm},m相似文献   

乳腺癌p21WAF1/CIP1表达与激素受体的相关性研究

目的 探讨乳腺癌p21WAF1/CIP1基因的表达与激素受体、肿瘤分级、淋巴结转移的关系。方法 应用免疫组化SP法对60例乳腺癌组织p21WAF1/CIP1基因及雌孕激素受体表达进行同时检测。结果 p21WAF1/CIP1、雌激素受体(ER)及孕激素受体(PR)阳性率分别为50%、55%、48%。p21WAF1/CIP1基因表达与ER、PR相关,并随着乳腺癌恶性程度增加和淋巴结转移阳性率降低。结论 检测p21WAF1/CIP1在乳腺癌的表达,对指导乳腺癌内分泌治疗和评估肿瘤生物学行为有重要意义。     

局部对称Lorentz流形中具常平均曲率的完备超曲面

设L1^n＋1是截面曲率KL满足b/2＜α≤KL≤b的局部对称Lorentz流形,M是L1^n＋1中具常平均曲率H的完备类空超曲面,S是M的第二基本形式模长平方,λ1,λ2……λn是M在点x处的n个主曲率,本文得到：如果L1^n＋1的截面曲率K（ei∧en＋1）满足∑λiK（ei∧en＋1）=nbH,则（i）S＜2√n-1（2a-b）时,M全脐：（ii）S=＜2√n-1（2a-b）时,若n=2,M全脐：若n≥3,M是双曲柱面。该结论是文[3]中相庆结果的推广与改进。