地下工程注浆技术研究新进展

注浆是土木、水利和矿山等工程建设中重要的防渗和加固技术。文章基于国内外注浆技术研究文献,总结了水泥等颗粒性浆液的“渗滤效应”及“黏度时空变异性”等理论研究成果,论述了非水反应高聚物、CW环氧树脂和微生物菌液等注浆新材料的应用现状及发展前景,介绍了细观力学数值模拟研究的突破性进展,可为地下工程注浆技术发展提供借鉴和启发作用。     

辽宁农业劳动生产率变动及影响因素分析

按照时间序列分析辽宁农业劳动生产率的变动趋势，运用人均耕地面积、灌溉率、劳均化肥施用量、结构调整系数、机电提灌面积占有效灌溉面积比重等指标构建影响农业劳动生产率的模型，结果表明农业产业结构调整、劳均化肥施用量、灌溉率对劳动生产率的影响非常显著．因此，应该加快农业产业结构调整、推进科学技术在农业生产中的应用、加强农业基础设施建设、提高公共服务水平．     

Synthesis of a new chitosan derivative and assay of Escherichia coli adsorption

A new chitosan derivative is prepared using chitosan.Ethyl chlorocarbonate was first introduced to the hydroxyl group of phthaloylchitosan through a nucleophilic reaction.Hydrazine was then added to recover the amino groups of chitosan and promote cross-l     

The clinical analysis of three methods in the treatment of intracranial bacterial infection

Objective To analyze the effect of three therapeutic methods to find an optimal approach to the treatment of intracranial bacterial infection by retrospectively reviewing 33 intracranial bacterial infection patients who were admitted from 1995 to 2008 in oar hospital. Methods The treatments by intermittent lumbar puncture,continuous lumbar subarachnoid space drainage, and embedment of Ommaya cyst for continuous drainage from the ventricles were performed in 15 cases, 12 cases, and 6 cases respectively along with intravenous application of full dose of antibiotics. Results Nineteen cases were cured and the best prognosis was from the group of Ommaya cyst embedment and continuous drainage from the ventricles. Conclusion Management goals are prompt recognition of the central nervous system (CNS) infection, rapid identification of causative organisms and initiation of treatment with the optimal management methods for complications. Embedment of Ommaya cyst for continuous drainage from the ventricle is a safe and effective treatment for intracranial bacterial infection.     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

自风湿性心脏病患者血液及骨髓中分离类星形念珠菌报告

本文报道一例临床诊断为风湿性心脏病的患者,7次自其血液,1次自其骨髓中分离出两株菌落形态不同的念珠菌,一株具有类星形念珠菌样菌落,另一株菌落不典型。经鉴定两株均为类星形念珠菌(Candida stellatoidea)。对家兔、豚鼠及小鼠作动物接种试验,证明两株均有较弱的致病力。这在国内外为首例报道。本实验结果表明类星形念珠菌像白色念珠菌、热带念珠菌等一样,可作为风湿性心脏病的继发感染菌,引起念珠菌性心内膜炎。临床上应予注意,必要时应采血作真菌学检验,以便作出正确诊断,进行合理治疗。     

多模式区域公交协调调度模型和算法

考虑公交内部及公交和地铁、长途客运之间的换乘衔接问题,满足最小和最大发车间隔等现实约束因素,建立了一类多目标多模式公交协调时刻表模型,在某时段内分别追求非换乘和换乘出行者在所有站点的等车时间最少和所有车辆到达站点时的泊位数最多.利用约束法将该问题转化为单目标规划问题.根据模型特征,设计求解该问题的改进细菌觅食优化算法,定义解的编码方案,设计产生初始种群的启发式算法,将梯度概念引入移动步长进而改进细菌觅食操作.最后,结合一个简单算例,比较单模式和多模式区域公交协调调度之间的差异,分析了站点通行能力对调度结果的影响,并将该算法与其他智能算法进行了比较分析,从而验证了模型和算法的正确性和有效性.     

腹腔外科感染的抗生素疗法

腹腔外科感染病人,抗生素疗法占有重要地位。252例腹腔脓液普通培养,24.2%无菌生长。培养出的菌丛中,绝大多数为革兰氏阴性细菌,大肠艾希氏菌占32.5%。选用抗生素应考虑:①菌丛及其联合存在的多样性;②药物进入胆汁的量与速度;③血与组织中的浓度;④药物的抗菌谱,毒性和价格。     

原产地规则研究

原产地规则是各国和地区为了确定商品原产国或地区而采取的法律、规章和普遍适用的行政命令;原产地规则有多种类别;原产地规则对全世界的和我国的国际贸易有多方面的影响和作用;现行中国原产地规则存在若干问题;我国关于原产地规则的对策可以从多方面加以完善.     

巨细胞病毒感染诱导移植排斥反应的机制研究

目的以人脐静脉内皮细胞(HUVECs)ECV304株作为研究对象,探讨巨细胞病毒(CMV)感染诱发排斥反应的机制。方法体外培养ECV304,施加不同干预因素,分为正常对照组、灭活病毒作用组、病毒作用组、病毒上清液作用组及病毒与更昔洛韦组。运用免疫组化技术检测不同组别内皮细胞表面细胞间粘附分子-1(ICAM-1)的表达。结果正常对照组内皮细胞ICAM-1的表达呈弱阳性,加入灭活的病毒及病毒上清液后,ICAM-1的表达仍为弱阳性(P>0.05);加入有活力的病毒后,内皮细胞ICAM-1的表达呈强阳性,积分指数显著高于上述3组(P<0.01);在CMV感染内皮细胞的同时加入更昔洛韦,细胞表面ICAM-1表达强度较病毒组并无减低(P>0.05)。结论CMV感染的内皮细胞表面ICAM-1的表达明显增加。通过诱导内皮细胞表面ICAM-1表达上调可能是CMV诱发排斥反应的机制之一。