T_H1 AND T_H2 CELL ANTIGEN RECEPTORS IN EXPERIMENTAL LEISHMANIASIS

Ｔ_Ｈ１ＡＮＤＴ_Ｈ２ＣＥＬＬＡＮＴＩＧＥＮＲＥＣＥＰＴＯＲＳＩＮＥＸＰＥＲＩＭＥＮＴＡＬＬＥＩＳＨＭＡＮＩＡＳＩＳＳｔｅｖｅｎＬ．Ｒｅｉｎｅｒ，ＷａｎｇＺｈｉ＇ｅｎ；ＦａｒａｈＨａｔａｍ，ＰｈｉｌｌｉｐＳｃｏｔｔ，ＲｉｃｈａｒｄＭ．Ｌｏｃｋｓｌｅｙ（...     

增殖细胞核抗原在胃癌中的表达与淋巴结转移、预后的关系

为探讨增殖细胞核抗原 ( Proliferating cell nuclear antigen,PCNA)在胃癌的表达与淋巴结转移及预后的关系 ,采用免疫组化法 ( ABC法 )测定 94例胃癌石蜡包埋标本。结果 :1胃粘膜不同病理状态下 PCNA指数差异显著 ( P <0 .0 1 ) ;2PCNA指数同患者的性别和年龄无关 ( P >0 .0 5 ) ,与肿瘤的生长方式、组织类型、浆膜层受侵与否、淋巴结转移与否和临床分期关系密切 ( P <0 .0 5 ) ;3PCNA指数与胃癌的预后密切相关 ( P <0 .0 5 ) ,弱级 ( 1～ 2级 )表达的患者预后较好 ,提示 PCNA检测可作为判断胃癌生物学行为和预后的一项良好指标。     

REPORT OF HLA DISTRIBUTION IN 2315 VOLUNTEER DONORS OF CHINESE BONE MARROW BANK FROM NORTHWEST CHINA

HumanLeukocyteAntigen(HLA)system, knownashumanmajorhistocompatibilitycomplex (MHC),playsakeyroleinantigenprocessing,im muneregulationandotheraspects.HLAisalsobeen calledastransplantingantigenasaccuratetypingof HLAonbothpatientanddonorcanincreasedramat i…     

改进动态克隆选择算法在邮件过滤中的应用研究

提出了可控变异和随机变异方法,改进了动态克隆选择算法,建立并实现一种改进的基于人工免疫系统的邮件过滤算法.并采用SpamAssassin垃圾邮件样本对算法性能进行测试.实验结果表明,引入改进的动态克隆选择算法后,该算法对具有变异特性的垃圾邮件能保持较高的正确识别率,具有较好的自适应性和多样性.     

TGFβ1-(78-109)-HBc融合蛋白的原核表达

目的　应用基因工程技术制备TGFβ1 (78- 10 9) HBc融合蛋白 ,作为TGFβ1疫苗 ,用于抗纤维化研究。方法　合成TGFβ1 (78- 10 9)编码区DNA ,连接于质粒载体 ,再在其下游连接HBc编码区 ,测序证实后 ,进行原核表达 ,表达产物以SDS PAGE、ELISA等方法鉴定。结果　经DNA序列分析证实融合基因序列完全正确 ,SDS PAGE证实原核表达产物相对分子质量为 2 5kD ,ELISA检测证实TGFβ1 (78- 10 9)和HBc的抗原表位均可正确暴露。结论　本研究成功构建了TGFβ1 (78- 10 9) HBc融合基因 ,进行了原核表达 ,并初步证实了表达产物的抗原性 ,为下一步进行TGFβ1疫苗抗纤维化的研究打下了基础     

EXPRESSION AND SIGNIFICANCE OF BASIC FIBROBLAST GWOWTH FACTOR AND FIBROBLAST GROWTH FACTOR RECEPTOR-1 IN OVARIAN EPITHELIAL NEOPLASM

Basicfibroblastgrowedfactor (bFGF)wasakindofmultifunctionalgrowthfactorwhichhadahighaffcinitywithitsmainreceptorwhosenamewasfibroblastgrowthfactorreceptor 1(FGFR 1) .ByFGFRsuchasFGFR 1,bFGFcouldnotonlyin ductthegrowthofnewcapillary ,butalsostimulatethecell’smitokasisandpropagationaspowerfulmi tosigen ;Bystimulatingendothelialandtumorcelltosynthesizetheprofibrnolysinactivator(uPA ) ,re leasefibrinaseandcollagenase ,synthesizecollage nase ,sotoenhencetumorcell’sabilitytoinvadeentracell…     

DETECTION OF CYTOMEGALOVIRUS（CMV） IMMEDIATE EARLY ANTIGENIN KIDNEY BIOPSIES AND TRANSPLANT NEPHRECTOMIES

Cytomegalovirus (CMV )infectionisoneofthemostcommonposttransplantcomplicationsandwasassociatedwithanincreasedmorbidityandmor talityintransplantrecipients[1,2 ] .Duringthefirst 4monthsafterrenaltransplantation ,theincidenceofactiveCMVinfectionisapproximately 5 0 % -90 % [3] .Recently ,manyresearchershavefoundthatCMVcouldaffecttheimmunityofhumanbe ing .CMVinfectionisalsoariskfactorforacuteandchronicrejectionofrenalallograftanddirectlycorrelateswithdecreased patientandallograftsur vival[4 - 7…     

EXPRESSIONS OF P53, PROLIFERATING CELL NUCLEAR ANITIGEN, BCL-2 PROTEIN AND THEIR SIGNIFICANCE IN SALIVARY ADENOID CYSTIC CARCINOMA

Studiesonthecarcinomasshowedthatthespeedofcarcinomadevelopmentisdependentonthebalanceofcellproliferatingandapoptosis.Soitisveryimportanttostudythemechanismbywhichoncogenescontrolproliferationandapoptosisoftu-morcell.Moststudiesonsalivaryglandtumorshavelaidemphsisontheproliferatingfunctionofonco-genesathomeandabroad,andignoranteditsfunc-tiononapoptosis,especially,thestudieswerefew-.erincombiningtwokindsoffunction.Helen's[13studyonACCindicatedthatmutationofP,3doesn'tplayimportantroleincarcinog…     

核受体免疫组织化学染色时抗原修复方法的选择

目的 探讨不同抗原修复法对DA.1U大鼠肝组织内核受体检测结果的影响.方法 应用微波修复法、高压修复法、酶修复法和高压加酶修复法对DA.1U大鼠肝组织切片进行修复,然后进行免疫组化染色.结果 DA.1U大鼠肝组织内核受体LXR-α、LXR-β、PPAR-γ和FXR经高压抗原修复后,染色阳性强度较其他修复方法明显增强,而且定位较佳.结论 在核受体免疫组化染色中高压抗原修复法染色效果优于其他抗原修复法.