TELEMEDICINE NETWORK BASED ON CODE-DIVISION MULTIPLE ACCESS WIRELESS TECHNOLOGY

Telemedicinehasbeenwidelyusedinmanyhealthcareapplicationssuchasmedicalcareinre moteruralareasandaftercareofpatientswithpace maker implanted[4] .Anotherwell knownexampleoftelemedicineistheremotetransmissionanddiag nosisofECGthroughtelephonenetwork[1 -3 ] .Telemedicineisalsoveryhelpfulinmanyemergen cyandambulancesituations.Earlydiagnosisbyaspecialistinemergenciesisparticularlyimportanttothepatients[5,6] .RecentstudyhasshownthatsocialchangesinJapanhaveresultedinarapidincreaseofacutecardiovascul…     

A Class of Singularly Perturbed Problems for Nonlinear Two-Species Competitive Reaction-Diffusion System

Wang[1-4]studiedthesingularlyperturbedprob lemsofellipticequationsandnonlinearequations,Mo[5]discussedaclassofnonlinearpredator preysin gularlyperturbedRobinproblemsforreaction diffu sionsystems.Thesingularlyperturbedproblemfor nonlineartwo speciescompeti…     

NT4-Ant-Shepherdin[79-87]融合基因重组载体的构建

目的设计构建携带NT4-Ant-Shepherdin[79-87]的cDNA融合基因的重组载体,为针对Survivin的靶向治疗奠定基础。方法应用非对称引物/模板法、PCR技术制备NT4-Ant-Shepherdin[79-87]cDNA片断,连接于pGEM-T-Easy载体,经克隆测序、酶切后与PBV220/NT4质粒连接;转化感受态细胞E.coliDH5α,亚克隆获得NT4-Ant-Shepherdin[79-87]融合基因。结果克隆出Ant-Shepherdin[79-87]基因,经酶切及测序证实结果正确;连接PBV220/NT4,经克隆、酶切,琼脂糖凝胶电泳证实获得321bp的NT4-Ant-Shepherdin[79-87]目的基因片断。结论通过分子生物学技术成功构建了携带NT4-Ant-Shepherdin[79-87]融合基因的重组载体,为进一步研究针对Survivin的靶向抗肿瘤作用奠定了基础。     

PRIMARY STUDY ON MECHANISM OF COLLAGEN SYNTHESIS INHIBITION IN CULTURED HUMAN FETAL HEPATOCYTES PRETREATED BY SALVIA MILTIORRHIZA BUNGE

Ithasbeenclearedthatreactiveoxygenspeciesandreactiveoxygenspecies inducedlipidperoxida tioncanstimulatecollagensynthesisofhepaticparenchymalcellsandnonparenchymalcellsandre sultinhepaticfibrosis,andthecollagensynthesisincreasingofHepatocytesmay playanimportantroleinhepaticfibrosis[1 -5] .Wehavefoundthatsalviamiltiorrhizabunge (SMB)couldprotecthu manfetalhepatocytesfromcarbontetrachloride(CCl4)mediatedlipid peroxidationinjuryinvit ro[6] .However,theeffectofSMBoncollagensyn thesisofhumanhepat…     

TRANSFORMATION OF EFFUSION AND FORMATION OF GRANULATION TISSUE IN PATHOLOGIC PROCESS OF OTITIS MEDIA WITH EFFUSION

Exceptforinaminorityofthecaseswithearlystageotitismedia (OM)orsequelae ,effusionisthemostcommoninflammatoryproductamongthevar iousOMtype .Itisnotclearwhetheruntreatedef fusionresultsinchronic ,irreversible pathologicchangesinthemiddleearcleftandmastoid[1 -4 ] .Chronicotitismedia (COM)ismainlyindicatedbytheformationofgranulationtissue .Granulationtis sueisthemostcommon [5,6] andsignificantrepresenta tivepathologictissueofCOM .Studiesonthefac tors promoting granulationtissueformation ,thepat…     

A Fast Attack Algorithm on the MD5 Hash Function

Introduction Cryptographichashfunctions[1-3]areimportant cryptographicprimitivesandusedinauthentica-tion,non-repudiation,electroniccommerceand encryptionschemes.Cryptographichashfunctions havetosatisfyrequirementsofonewaynessand collisionresistance[4-7].Onesuchfamilyofhash functionsistheMDxfamily.Thisfamilyincludes hashfunctionssuchasMD5,SHA-1and RIPEMD-160.TheMD5hashfunctionwasproposedby Rivest[2].AnattackonMD5waspresentedby Wang[8].Butthedetailofattackalgorithmwasnot discovereduntil…     

THE APPLICATION OF STATISTICAL PARAMETERS OF PHASE RESOLVED PD DISTRIBUTION TO AGING EXTENT ASSESSMENT OF LARGE GENERATOR INSULATION

Agingextentassessmentofgeneratorinsulationisofgreatsignificanceinplanninganeffectiveout ageandmaintenance .Inthelastdecade ,agingex tentassessmentoflargegeneratorinsulationwasasubjectresearchedintensively[1-4] .Anumberofpa pershavebeenpublishedonthetechniquesofinsu lationconditiondiagnosisandagingextentassess mentofgeneratorinsulation .Nearlyalleffortsforinsulationdiagnosisandagingassessmenthavereliedonelectricalmeasurementsandmostattentionhasbeenpaidtoanalysisofcharacteristicsofpartialdis cha…     

A Secret Sharing Featured Secure Communication System Based on Dual Synchronization of Chaos in Colpitts Circuits

Introduction Synchronizationofchaoshasrecentlyattract-edgreatinterestbecauseofitspotentialapplica-tionsinsecurecommunications[1]andspreadspec-trumcommunications.Inasecurecommunication system,itcanbeusedforsharingtheidentical chaosinthetransmitterandreceiverasacrypto-graphiccode.Synchronizationofchaosinonewaycoupled onepairchaoticcircuitshasbeenstudiedinalarge volumeofliteratures[2-4].Inrecentyears,anew conceptofchaossynchronization,namely“dual synchronizationofchaos”,israised[5].Dualsyn-chr…     

噻并噻吩-苯并二噻吩和TPTI共聚物的光伏性能

通过Stille聚合反应合成了含有烷基噻并[3,2-b]噻吩修饰的苯并[1,2-b∶4,5-b′]二噻吩(BDT)和五元稠环噻吩[2′,3′∶5,6]吡啶并[3,4-g]噻吩[3,2-c]异喹啉-5,11(4H,10H)-二酮(TPTI)单元D-A型共聚物PTTBDTTPTIC12和PTTBDT-TPTIHD,对比研究发现:通过改变和增加聚合物侧链的形状和大小,可增加聚合物的溶解性,改善器件的溶液加工性.基于倒置型光伏器件的测试结果显示:溶解性好的聚合物PTTBDT-TPTIHD的光伏性能较好,以聚合物PTTBDT-TPTIHD为给体、PC71BM为受体、MoO_3为缓冲层、Ag为阳极制备了本体异质结聚合物太阳能电池,当给受体质量比为1∶1.5时,能量转换效率(PCE)达到了3.09%,其开路电压(VOC)为0.83V;短路电流密度(JSC)为6.98mA·cm~(-2);填充因子(FF)为53.34%.     

FABRICATION AND CHARACTERATION OF NANOPOROUS SILICA FILM

Microporoussilicafilmswithspecialpropertiesareexpectedtobeusedinmanyfields,suchasopticalcoat ing[1 ] ,sensor,membrance[2 ] ,anddielectricfilm[3 ] .Itslowsoundspeedandacousticdelayorhigherratiosoundinsulatingmaybeusedasultrasounddetector[4] .Theexcellentfeaturesofinsulatingpropertyanditsnearper fectinterfaceonsiliconcouldplayakeyroleinsuccessofverylargescaleintegrated(VLSI)devices[5\〗,etc.Intheotheraspects,nanoporoussilicafilmsarebeingusedinseparationtechnology[6] ,molecularengineering,andbi…     

移植肾排异彩色多普勒血流显像与肾穿刺活检的对照研究

目的　分析彩色多普勒血流显像 (CDFI)与肾活检在移植肾排异中的作用 ,评估无创性CDFI对移植肾术后的诊断价值。方法　对 5 9例肾移植术后患者行CDFI检查并参照病理诊断 ,对二维图像、彩色血流图、血流频谱及阻力指数 (RI)、搏动指数 (PI)、收缩期与舒张期血流速度比 (S/D)等参数进行分析。结果　急性排异组的RI值为0 .77± 0 .0 5 ,PI值为 1.4 7± 0 .33,S/D为 4 .0 1± 1.33;慢性排异组的RI值为 0 .72± 0 .0 7,PI值为 1.2 2± 0 .5 1,S/D为 2 .90± 1.0 1,两组RI值、PI值、S/D明显高于正常组 (RI值 0 .5 7± 0 .0 7;PI值 0 .87± 0 .2 8;S/D为 2 .33± 0 .4 2 )。手术后早期急性排异患者CDFI可靠评价移植肾低灌注 ,RI <0 .72。结论　CDFI监测移植肾后的排异反应快速 ,准确无创 ,可早期发现病情变化并指导治疗     

RELATIONSHIP BETWEEN CYTOMEGALOVIRUS INFECTION AND THE PRODUCTION OF AN TICARDIOLIPIN ANTIBODY IN RENAL TRANSPLANT RECIPIENTS

Ｔｈｅｉｎｆｅｃｔｉｏｎｏｆｃｙｔｏｍｅｇａｌｏｖｉｒｕｓ(ＣＭＶ )ｉｓｏｎｅｏｆｔｈｅｍｏｓｔｓｅｒｉｏｕｓｃｏｍｐｌｉｃａｔｉｏｎｓａｆｔｅｒｒｅｎａｌｔｒａｎｓ ｐｌａｎｔａｔｉｏｎ[1] .ＭｏｓｔｂｅｌｉｅｖｅｔｈａｔＣＭＶｉｎｆｅｃｔｉｏｎｎｏｔｏｎｌｙｃａｎｉｎｄｕｃｅａｃｕｔｅｒｅｊｅｃｔｒｅａｃｔｉｏｎｏｆｔｈｅｔｒａｎｓｐｌａｎｔ ,ｂｕｔａｌｓｏｐｌａｙｓａｒｏｌｅｉｎｔｒａｎｓｐｌａｎｔａｔｉｏｎ ｒｅｌａｔｅｄａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ[2 ] .Ａｓａｒｅｓｕｌｔ ,ｉｔａｆｆｅｃ…     

STUDY ON B7-1 PROTEIN‘S EXPRESSION IN BIOPSIES IN RENAL TRANSPLANTATION

Ａｌｌｏｇｒａｆｔｒｅｊｅｃｔｉｏｎｉｓａｎｉｍｐｏｒｔａｎｔｆａｃｔｏｒｏｆｉｎ ｆｌｕｅｎｃｉｎｇｔｈｅｌｏｎｇｔｅｒｍｓｕｒｖｉｖａｌｏｆａｌｌｏｇｒａｆｔｉｎｈｕ ｍａｎｏｒｇａｎｔｒａｎｓｐｌａｎｔａｔｉｏｎ .Ｉｍｍｕｎｏｌｏｇｉｃａｌｔｏｌｅｒａｎｃｅｉｎｄｕｃｔｉｏｎｉｓａｍａｉｎ ｐａｒｔｉｎｔｈｅｒｅｓｅａｒｃｈｏｆｏｒｇａｎｔｒａｎｓｐｌａｎｔａｔｉｏｎ .Ｔｏｓｔｕｄｙｔｈｅｉｍｍｕｎｏｌｏｇｉｃａｌａｎｄｍｏｌｅｃｕｌａｒｍｅｃｈａｎｉｓｍｏｆｒｅｊｅｃｔｉｏｎａｎｄｓｅｅｋ…     

人胃肠癌组织中浸润淋巴细胞活性的原位分析

利用一组抗淋巴细胞亚群的单克隆抗体和免疫组化方法对63例进展期胃癌和肠癌组织中浸润单个核细胞,尤其是散在浸润淋巴细胞(主要是T淋巴细胞)的I_2(HLA—DR)抗原和OKT_9抗原的表达进行了分析。同时,无选择的选取了18例慢性胃炎和慢性肠炎活检标本做为对照。在癌肿标本中,散在浸润淋巴细胞的I_2抗原表达显著变异。少数标本中很难查见I_2~+T细胞,大部分病例只能查见少数细胞呈I_2~+。63例中仅有10例其I_2~+细胞占全部散在浸润淋巴细胞的10—30%,所有瘤组织中几乎无OKT_9~+浸润T细胞。这种表现完全不同于正常粘膜及炎症粘膜中的I_2~+浸润单个核细胞分布。这说明就大多数病例来说,肿瘤组织中浸润的T细胞多数是非活化的。     

ROLE OF PERIPHERAL LYMPHOCYTES SIALYL LEWIS（X） （CD15s） ANTIGEN BEFORE AND AFTER KIDNEY TRANSPLANTATION

Objective To investigate the role of peripheral lymphocytes Sialyl Lewis（x） （CD15s） antigen before and after kidney transplantation. Methods Flow cytometry technique was applied to examine the expression of peripheral lymphoid cell surface CD15s antigen after renal transplantation, and to evaluate various therapeutic regimen. Results The statistic analysis results of peripheral lymphoid cell surface CD15s antigen expression level showed that there was significant difference among the patients with acute rejection, long-term dialysis and with normal renal function post-transplant; significant difference of CD15s expression level between group of rejection and infection; no significant difference of CD15s expression among the different groups treated by various therapeutic regimens. Conclusion The different therapeutic regimen has no influence to CD15s expression; Detection of peripheral lymphoid cell surface CD15s antigen expression periodically, intelligently make convenience to understand suitable status of immunosuppression.     

肝移植术后巨细胞病毒感染的诊断和治疗

目的 分析肝移植术后受体巨细胞病毒(CMV)感染的诊断和治疗.方法 回顾性分析我科2000年11月至2007年12月期间进行的肝移植病例,依据易感因素、临床症状、外周血中CMV-PP65和(或)CMV-IgM及胸片明确诊断,以更昔洛韦抗病毒治疗.结果 共收集资料111例(剔除相关资料不完整者).发现CMV感染5例,CMV感染发生率为4.5%;其中2例发生CMV肺炎,CMV肺炎发生率为1.8%(占CMV感染的40%),2例均好转.活动性CMV感染3例,2例治愈,1例好转.结论 CMV-PP65检测能够对CMV感染患者做出早期诊断并且指导治疗.更昔洛韦能够有效治疗CMV感染.     

巨细胞病毒感染诱导移植排斥反应的机制研究

目的以人脐静脉内皮细胞(HUVECs)ECV304株作为研究对象,探讨巨细胞病毒(CMV)感染诱发排斥反应的机制。方法体外培养ECV304,施加不同干预因素,分为正常对照组、灭活病毒作用组、病毒作用组、病毒上清液作用组及病毒与更昔洛韦组。运用免疫组化技术检测不同组别内皮细胞表面细胞间粘附分子-1(ICAM-1)的表达。结果正常对照组内皮细胞ICAM-1的表达呈弱阳性,加入灭活的病毒及病毒上清液后,ICAM-1的表达仍为弱阳性(P>0.05);加入有活力的病毒后,内皮细胞ICAM-1的表达呈强阳性,积分指数显著高于上述3组(P<0.01);在CMV感染内皮细胞的同时加入更昔洛韦,细胞表面ICAM-1表达强度较病毒组并无减低(P>0.05)。结论CMV感染的内皮细胞表面ICAM-1的表达明显增加。通过诱导内皮细胞表面ICAM-1表达上调可能是CMV诱发排斥反应的机制之一。     

DETECTION AND SIGNIFICANCE OF HBV IN RENAL TISSUE OF HBV ASSOCIATED GLOMERULONEPHRITIS PATIENTS

ＳｉｎｃｅＣｏｍｂｅｓ[1] ｆｉｒｓｔｌｙｒｅｐｏｒｔｅｄｏｎｅｃａｓｅｏｆｔｈｅＨＢＶ ａｓｓｏｃｉａｔｅｄｍｅｍｂｒａｎｏｕｓｎｅｐｈｒｏｐａｔｈｙ (ＨＢＶ ｍＧＮ )ｉｎ 1971,ｔｈｅｒｏｌｅｏｆｈｅｐａｔｉｔｉｓＢｖｉｒｕｓ (ＨＢＶ)ｐｌａｙｅｄｉｎ ｇｌｏｍｅｒｕｌｏｎｅｐｈｒｉｔｉｓｗａｓ ｇｅｎｅｒａｌｌｙ ｐａｉｄｃｌｏｓｅａｔｔｅｎｔｉｏｎｗｈｅｎｉｔｓｍｅｃｈａｎｉｓｍｗａｓｉｎｖｅｓｔｉｇａｔ ｅｄ .Ｉｔｒｅｍａｉｎｓｕｎｃｅｒｔａｉｎａｔｐｒｅｓｅｎｔ,ｈｏｗｅｖｅｒ ,ｈｏｗＨＢＶｐｌ…     

猪鼻支原体P40蛋白在肾癌组织中的表达及意义

目的检测猪鼻支原体P40蛋白在肾癌组织中的表达,探讨支原体感染与肾癌发生的关系。方法选取本院肾癌石蜡包埋标本95例,以远离肾癌的正常肾组织标本60例为对照,采用免疫组织化学方法检测肾组织中猪鼻支原体P40蛋白的表达情况。结果肾癌组织中猪鼻支原体P40蛋白阳性表达率为64.2%(61/95);癌旁正常肾组织的阳性表达率为21.7%(13/60),两者相比差异具有显著统计学意义(P<0.001)。结论肾癌的发生与支原体感染有一定的相关性,支原体感染可能是肾癌的生物学致病因素。     

狼疮性肾炎肾活检组织中PCNA与Bcl-2的表达及意义

目的　观察狼疮性肾炎 (lupusnephritis ,LN)肾组织内凋亡调控基因Bcl 2及增殖细胞核抗原 (PCNA)的表达 ,探讨凋亡在LN发病中的作用。方法　应用ELPS(enhancedlabelled polymersystem)检测 2 9例LN肾穿刺活检组织PCNA和Bcl 2的表达 ,并与正常组进行对比分析。结果　LN组肾小球内PCNA阳性细胞数与正常对照组肾组织比较 ,有显著性差异 (P <0 .0 0 1)。LN组肾小球内Bcl 2阳性表达细胞明显高于正常对照组 (P <0 .0 0 1) ,阳性表达细胞见于系膜细胞、毛细血管内皮细胞和细胞性新月体内。LN组肾小球Bcl 2阳性细胞数与PCNA阳性细胞数、肾小球细胞总数有显著正性线性相关 (r =0 .70 1.P <0 .0 1;r =0 .5 5 0 ,P <0 .0 1)。结论　LN肾组织肾小球内细胞高增殖和Bcl 2高表达 ,提示细胞增殖与凋亡调控异常均参与了LN肾小球的增生性病变